淡水鱼中隐性孔雀石绿的快速提取和检测  

Rapid extraction and analysis of leuco malachite green in fresh water fish

作　　者：刘柳[1] 徐传杰 韦媛媛[1] 林华 忻欣 韩莹 粟晖[1] LIU Liu;XU Chuan-jie;WEI Yuan-yuan;LIN-hua;XIN Xin;HAN Yin;SU Hui(Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology,LiuZhou 545006,China;Guangxi Zhuang Autonomous Region Institute of Metrology & Test,NanNing 530007,China;Liuzhou Institue for Food and Drug Control,LiuZhou 545006,China;CSEPAT(Beijing)Technology Co.,Ltd.,Beijing 100029,China)

机构地区：[1]广西糖资源绿色加工重点实验室,广西科技大学,广西柳州545006 [2]广西壮族自治区计量检测研究院,广西南宁530007 [3]柳州市食品药品检验所,广西柳州545006 [4]西派特(北京)科技有限公司,北京100029

出　　处：《化学研究与应用》2020年第7期1170-1175,共6页Chemical Research and Application

基　　金：广西重点研发计划(桂科AB16380348)资助。

摘　　要：基于向量-子空间夹角判据,建立了淡水鱼中隐性孔雀石绿(LMG)的快速提取和紫外光谱检测方法。首先采用高效液相色谱-紫外可见光谱仪联用,建立不含待测组分LMG的淡水鱼本底数据库;然后采集LMG的标准光谱数据库,即可建立快速分析的数学模型。运用该模型,直接采集市售淡水鱼的紫外可见光谱即可快速计算LMG的含量。实验结果表明:快速分析法和高效液相色谱法结果接近,加标回收率在92~120%之间;乙腈提取法的提取率高于国标法,相对标准偏差小于5%,重现性好,解决了现有隐性孔雀石绿提取方法繁琐的问题;实现了淡水鱼中隐性孔雀石绿快速提取和检测。A rapid extraction and UV analytical method of LMG was established based on the criterion of vector-subspace.Firstly,the sample background spectrum database was constructed and separated by HPLC-UV,which does’t contain LMG spectra matrix deducted from the sample spectrum database.Secondly,collecting LMG standard spectrum.Then the mathematics model was constructed with the sample background matrix and LMG standard spectrum to determine LMG in fresh water fish by collecting the UV spectrum of sample.The study showed that the results of rapid analytical method were close to HPLC,the recovery rates were 92%~120%;the extraction rates with Acetonitrile were better than the national standard method,which the relative standard deviation of rapid pretreatment was within 5%with good reproducibility.This method was effective for rapid detection of LMG in fresh water fish and solved the problems of complex operation.

关 键 词：隐性孔雀石绿 孔雀石绿 向量-子空间夹角 紫外可见光谱 

分 类 号：O657.3[理学—分析化学]