好氧反硝化细菌的鉴定及其脱氮特性研究  

IDENTIFICATION AND DENITRIFICATION CHARACTERISTICS OF THE AEROBIC DENITRIFYING BACTERIA PSEUDOMONAS FURUKAWAII ZS1

作　　者：朱云 龚望宝[1] 谢骏[1] 王广军[1] 余德光[1] 李志斐[1] 张凯[1] 田晶晶 ZHU Yun;GONG Wang-Bao;XIE Jun;WANG Guang-Jun;YU De-Guang;LI Zhi-Fei;ZHANG Kai;TIAN Jing-Jing(Key Laboratory of Tropical&Subtropical Fishery Resource&Cultivation,Ministry of Agriculture and Rural,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院珠江水产研究所,农业农村部热带亚热带水产资源利用与养殖重点实验室,广州510380 [2]上海海洋大学,水产与生命学院,上海201306

出　　处：《水生生物学报》2020年第4期895-903,共9页Acta Hydrobiologica Sinica

基　　金：中国水产科学研究院中央级公益性科研院所基本科研业务费专项(2018SJ-YB01);广东省促进经济发展专项资金(现代渔业发展用途)省级科技创新项目(粤农2019B13);国家自然科学基金(31302201);国家现代农业产业技术体系建设专项(CARS-45-21);广东省自然科学基金(2018A030313412)资助。

摘　　要：2018年3月,在广东省中山市的草鱼(Ctenopharyngodon idellus)养殖池塘水体中分离筛选出一株高效好氧反硝化细菌,结合菌株的形态观察、生理生化特性和16S rDNA基因序列分析鉴定为Pseudomonas furu-kawaii,命名为ZS1。并进-一步研究了该菌株的脱氮特性,同时采用单因素实验方法探究了不同碳源种类、温度、pH、CN和摇床转速对菌株ZS1脱氮效率的影响。结果表明,ZS1菌株在好氧条件下具有高效的脱氮效率。经过48h的培养,硝酸盐氮(NO3-N)的含量从48.93降低到1.27 mg/L,去除率为97.40%,去除速率达0.993 mg/(Lh);总氮(TN)的含量从5204降低到840 mg/L,去除率为83.86%,去除速率达0.909 mg/(L-h),呒亚硝酸盐氮(NO;-N)积累。以NO3-N为唯一氮源,菌株ZS1发挥最佳好氧反硝化性能的碳源为乙酸钠、柠檬酸钠和葡萄糖、温度为25-35℃、pH为7.0-10.0、CN为15--25、转速为100-200r/min。上述结果显示,菌株P.furukanwaiZS1具有良好的好氧反硝化性能,将为池塘养殖尾水处理应用生物脱氮技术提供理论依据及成为初步的候选菌株。An efficient aerobic denitrifying bacteria species was isolated from water in a Ctenopharyngodon idellus culture pond in Zhongshan City, Guangdong Province in March 2018. The bacteria were identified as Pseudomonas furukawaii by morphological observation, physiological and biochemical characteristics, and 16 S rDNA sequence analysis.This strain was named Pseudomonas furukawaii ZS1. The denitrification characteristics of the strain and the effects of the carbon source, temperature, pH, C/N ratio and shaking speed on the denitrification ability of strain ZS1 were investigated. The results showed that P. furukawaii ZS1 was an efficient denitrifier under aerobic conditions. The content of-N was 97.40%,and the removal rate was 0.993 mg/L/h;the content of total nitrogen(TN) decreased from 52.04 mg/L to 8.40 mg/L, the removal efficiency was 83.86%, and the removal rate was 0.909 mg/L/h. Single-factor experiments indicated that the optimal conditions for denitrification activity in P. furukawaii ZS1 were a carbon source of sodium acetate, sodium citrate or glucose;temperatures ranging from 25 to 35℃;a pH of 7.0 to 10.0;a C/N ratio of 15 to 25;and a shaking speed of 100 to 200 r/min. The above results showed that the strain could quickly and effectively remove nitrogen through aerobic denitrification. Therefore, the P. furukawaii ZS1 strain can be used as a candidate strain for the treatment of pond culture wastewater through biological denitrification.

关 键 词：好氧反硝化细菌 脱氮性能 环境因子 池塘养殖 

分 类 号：Q938.8[生物学—微生物学]