城镇污水处理系统中4种抗生素抗性基因的沿程变化  

Variations of 4 Antibiotic Resistance Genes in a Sewage Treatment Plant

作　　者：包樱钰 谢辉 陈吕军[2] 温东辉[1] BAO Yingyu;XIE Hui;CHEN Lüjun;WEN Donghui(College of Environmental Sciences and Engineering,Peking University,Beijing 100871;School of Environment,Tsinghua University,Beijing 100084)

机构地区：[1]北京大学环境科学与工程学院,北京100871 [2]清华大学环境学院,北京100084

出　　处：《北京大学学报（自然科学版）》2020年第4期772-776,共5页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金(51678003,51678334)资助。

摘　　要：为探究抗生素抗性基因(ARGs)在城镇污水处理系统中的沿程变化,针对4种关注度较高的ARGs(sulII,ermB,tetC和blaPSE-1)和I型整合子的整合酶基因(intI1),选取京津冀地区一座中小城镇的污水处理厂,使用普通PCR和实时荧光定量PCR技术,对各工艺单元的水样进行细胞内ARGs和intI1的定性及定量检测分析。结果表明,4种ARGs和intI1普遍存在于城镇污水处理系统中,出水中ARGs和intI1的绝对丰度降低,去除量在1.26~2.30 logs之间。相关性分析表明,intI1和水质因子(pH,COD和NH3-N)可能与4种ARGs的传播扩散有关。In order to understand the variations of antibiotic resistance genes(ARGs)in sewage treatment plants(STPs),the distribution and removal efficiencies of 4 subtypes of intracellular ARGs(sulII,ermB,tetC and blaPSE-1)and class I integron integrase gene(intI1)in a middle-scale STP in Hebei Province were detected by PCR and realtime fluorescent quantitative PCR(qPCR).4 ARGs and intI1 were found in all water samples and 1.26-2.30 orders of magnitude of ARGs were removed by the STP.Correlation analysis showed that intI1 and water quality factors including pH,COD,and NH3-N might affect the distribution and diffusion of tetC,ermB,and blaPSE-1.The final effluent of a STP may promote the spread of ARGs in surface water system.

关 键 词：抗生素抗性基因(ARGs) 城镇污水处理系统 A^2/O工艺 实时荧光定量PCR 

分 类 号：X703[环境科学与工程—环境工程]