Study of Soil Microbiological Properties  

作　　者：Hamid Kheyrodin 

机构地区：[1]Semnan University,Semnan,Iran

出　　处：《Journal of Chemistry and Chemical Engineering》2020年第2期66-72,共7页化学与化工（英文版）

基　　金：We thank Semnan University Department of Desert Science for supplying soil from Semnan Agriculture Station and Semnan University.

摘　　要：Two strategies have been developed to improve DNA recovery in terms of yield,purity and unbiased representation of the microbial diversity.However,amplification of DNA from soil is often inhibited by co-purified contaminants.Furthermore,DNA is also suitable for PCR amplification using various DNA targets.This review presents an overview of the available methods to achieve this challenging objective.DNA was extracted from 100 g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation,polyethylene glycol precipitation,phenol extraction and isopropanol precipitation.

关 键 词：Purification soil DNA RNA. 

分 类 号：O64[理学—物理化学]