乌梅、金银花、忍冬藤对嗜麦芽窄食单胞菌的抑菌作用  

Bacteriostatic activities of Dark Plum Fruit,Lonicera japonica and Caulis Lonicerae Japonicae for Stenotrophomonas maltophilia

作　　者：左惠芬[1] 吕国平 谷敬欣[1] 张丽杰[3] 英一铭[1] 王香玲[1] 吴淑慧 ZUO Huifen;LYU Guoping;GU Jingxin;ZHANG Lijie;YING Yiming;WANG Xiangling;WU Shuhui(Department of Clinical Laboratory,Hebei Yiling Hospital,Shijiazhuang,Hebei,050091;School of Basic Medicine,Hebei University of Traditional Chinese Medicine,Shijiazhuang,Hebei,050090;Department of Clinical Laboratory,The Third Hospital of Hebei Medical University,Shijiazhuang,Hebei,050051)

机构地区：[1]河北以岭医院检验科,河北石家庄050091 [2]河北中医学院基础医学院,河北石家庄050090 [3]河北医科大学第三医院检验科,河北石家庄050051

出　　处：《实用临床医药杂志》2020年第12期80-83,共4页Journal of Clinical Medicine in Practice

基　　金：河北省中医药管理局科研计划项目(2015214);河北省科技厅资助项目(17277743D)。

摘　　要：目的探讨乌梅、金银花、忍冬藤体外抑制嗜麦芽窄食假单胞菌的作用。方法选用纸片扩散法筛选对环丙沙星等12种抗菌药物全耐药的嗜麦芽窄食假单胞菌菌株;选用MH琼脂稀释法测定乌梅、金银花、忍冬藤对嗜麦芽窄食假单胞菌的抗菌效果,比较3种中药的最低抑菌浓度(MIC)、抑制50%受试菌所需MIC(MIC 50)、抑制90%受试菌所需MIC(MIC 90)。结果本研究共筛选出对环丙沙星等12种抗菌药物全部耐药的嗜麦芽窄食假单胞菌菌株30株;乌梅、金银花、忍冬藤对耐药嗜麦芽窄食假单胞菌均有一定的抑菌作用,其中乌梅抑菌作用较强。结论乌梅、金银花、忍冬藤对嗜麦芽窄食假单胞菌均有不同程度的抑菌作用,其中乌梅的抑菌作用最强,提示中药在耐药菌治疗的研究价值值得进一步研究。Objective To explore the inhibitory effects of Dark Plum Fruit,Lonicera japonica and Caulis Lonicerae Japonicae on Stenotrophomonas maltophilia in vitro.Methods Disc diffusion method was used to screen Stenotrophomonas maltophilia strains that had resistance to 12 kinds of antimicrobial agents(including ciprofloxacin).MH agar dilution method was used to determine the antibacterial effects of Dark Plum Fruit,Lonicera japonica and Caulis Lonicerae Japonicae on strains of Stenotrophomonas maltophilia.The minimum inhibitory concentration(MIC),MIC required to inhibit 50%of tested bacteria(MIC 50)and MIC required to inhibit 90%of tested bacteria(MIC 90)of three traditional Chinese medicine were compared.Results A total of 30 strains of Stenotrophomonas maltophilia that had resistance to all 12 antimicrobial agents were screened out.Dark Plum Fruit,Lonicera japonica and Caulis Lonicerae Japonicae all had antibacterial effects,among which Dark Plum Fruit had the best bacteriostatic effect.Conclusion Dark Plum Fruit,Lonicera japonica and Caulis Lonicerae Japonicae have different antibacterial effects against Stenotrophomonas maltophilia.Among them,the Dark Plum Fruit has the strongest antibacterial effect,which reveals that traditional Chinese herbs are worthy of further exploration in application value in the treatment of drug-resistance bacteria.

关 键 词：中草药 乌梅 金银花 忍冬藤 嗜麦芽窄食单胞菌 微生物敏感性试验 

分 类 号：R378.99[医药卫生—病原生物学]