思茅山橙中的单萜吲哚生物碱类成分  

Chemical investigation on monoterpenoid indole alkaloids of Melodinus henryi

作　　者：何隽[1] 张发雷 望石琴 王淙薇 崔琴丹 冯涛[1] HE Juan;ZHANG Falei;WANG Shiqing;WANG Congwei;CUI Qindan;FENG Tao(School of Pharmaceutical Sciences,South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2020年第4期370-375,共6页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(81872762)。

摘　　要：利用硅胶柱色谱、高效液相色谱、Sephadex LH-20凝胶柱色谱等方法从思茅山橙(Melodinus henryi)中分离纯化得到6个生物碱类化合物.通过UV、IR、NMR、MS等波谱学数据和理化性质鉴定结构分别是:19R-acetoxytabersonine(1)、11-methoxy-19-hydroxytabersonine(2)、16β-hydroxy-19R-vindolinine(3)、19R-kitramine(4)、trichophylline(5)、voaharine(6).化合物6的结构进一步被X-射线晶体衍射分析确证.以上化合物均为首次从该植物中分离得到.利用MTT法检测了化合物1~6对5种人类肿瘤细胞系的细胞毒活性,结果显示化合物1和2对5种人类肿瘤细胞系具有较好的细胞毒活性.Six monoterpenoid indole alkaloids were isolated from Melodinus henryi by using gel column chromatography,Sephadex LH-20 and HPLC.The structures were elucidated to be 19R-acetoxytabersonine(1),11-methoxy-19-hydroxytabersonine(2),16β-hydroxy-19R-vindolinine(3),19R-kitramine(4),trichophylline(5),and voaharine(6)on the basis of spectroscopic analysis(UV,IR,NMR,MS)and comparison with data reported in the literatures.Compound 6 was further confirmed by the single crystal X-ray diffraction.All compounds were isolated from this plant for the first time.All compounds were evaluated for their cytotoxicity against five human cancer cell lines.The results showed that compounds 1 and 2 displayed certain cytotoxicities against five human cancer cell lines.

关 键 词：思茅山橙 化学成分 生物碱 结构鉴定 

分 类 号：O692.3[理学—化学] R914[医药卫生—药物化学]