Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation  

作　　者：Jelena Bebic Katarina Banjanac Marija Corović Ana Milivojevic Milica Simovic Aleksandar Marinkovic Dejan Bezbradica 

机构地区：[1]Directorate of Measures and Precious Metals,Mike Alasa 14,11000 Belgrade,Serbia [2]Innovation Centre of Faculty of Technology and Metallurgy,University of Belgrade,Karnegijeva 4,11000 Belgrade,Serbia [3]Department of Biochemical Engineering and Biotechnology,Faculty of Technology and Metallurgy,University of Belgrade,Karnegijeva 4,11000 Belgrade,Serbia [4]Department of Organic Chemistry,Faculty of Technology and Metallurgy,University of Belgrade,Karnegijeva 4,11000 Belgrade,Serbia

出　　处：《Chinese Journal of Chemical Engineering》2020年第4期1136-1144,共9页中国化学工程学报（英文版）

基　　金：the Ministry of Education,Science and Technological Development,Republic of Serbia,within projectsⅢ46010,Ⅲ45019 and TR31035 for the financial;Directorate of Measures and Precious Metals,Ministry of Economy,Republic of Serbia for the technical support。

摘　　要：This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oryzae(Novozym 51003?laccase)on amino modified fumed nano-silica(AFNS)and the possible use in bioremediation.Hereby,for the first time,factors affecting the immobilization of Novozym 51003?laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization.The highest specific activity(13.1 IU·mg-1 proteins)was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield,reaching68.3%after the equilibrium time,at optimum pH 5.0,was obtained.Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min,following pseudo-first-order kinetics.The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane.Within 24 h,lindane concentration was reduced to 56.8%of initial concentration and after seven repeated reuses it retained 70%of the original activity.

关 键 词：Silica nanoparticles LACCASE Enzyme immobilization LINDANE BIOREMEDIATION 

分 类 号：X505[环境科学与工程—环境工程] TQ426.97[化学工程]