Effects of theophylline/chitosan/β-cyclodextrin microspheres for oral drug delivery on an asthmatic rat model  

作　　者：Saisai Wang Haijing Zhang Xiaoming Cui Liping Zhao Mingxia Jiang Zhilu Xu Dongzhen Mu Weifen Zhang 

机构地区：[1]School of Pharmacy,Weifang Medical University,Weifang,Shandong 261053,China [2]Intensive Care Unit,The First people’s hospital of Hefei,Hefei,Anhui 230061,China [3]Department of Immunology,Key lab for Immunology in Shandong Province,School of clinical Medicine,Weifang Medical University,Weifang,Shandong 261053,China [4]Institute for Smart Materials and Regenerative Medicine,Weifang Medical University,Weifang,Shandong 261053,China [5]Shandong Engineering Research Center for Smart Materials and Regenerative Medicine,Weifang Medical University,Weifang,Shandong 261053,China

出　　处：《TMR Modern Herbal Medicine》2020年第2期66-76,共11页TMR现代中药

基　　金：the foundation for visiting scholar abroad in Weifang Medical University,National Natural Science Foundation of China(No.81973671,81774125);Weifang Science and Technology Development Plan Project(2018YX060).

摘　　要：Objective:This study aimed to investigate the effects of TH/CTS/β-CD microspheres by oral administration on ovalbumin allergic asthma of a rat model.Methods:Spray drying method was applied for preparing the TH/CTS/β-CD microspheres,which were used in treatment of asthmatic rats.At a predetermined time,the levels of eosinophil(Eos),protein,lactate dehydrogenase(LDH),aspartate transaminase(AST),glutamic pyruvic transaminase(GPT),and creatinine(Cr)were ascertained by automatic hematology and biochemical analyzers.Lung tissue histology was performed by hematoxylin-eosin staining.Results:No significant differences were found about the contents of Eos in the blood,and the contents of Eos,protein and LDH in the bronchoalveolar lavage fluid between the microsphere-treated and dexamethasone-treated groups,but the levels were lower in both treated groups than in the model group.In the microsphere-treated group,the levels of Cr,AST,and GPT in the serum of rats showed no significant differences compared with the normal group.Based on lung histopathological findings,the microspheres attenuated inflammatory cell infiltration and mucus hypersecretion compared with the model group.Conclusion:This study demonstrated that TH/CTS/β-CD microspheres exerted an anti-inflammatory effect and could serve as a novel promising drug delivery system for asthma treatment.

关 键 词：CHITOSAN THEOPHYLLINE Asthma MICROSPHERES PHARMACODYNAMICS 

分 类 号：R56[医药卫生—呼吸系统]