Identification of plant genes putatively involved in the perception of fungal ergosterol-squalene^∞  

作　　者：Laura Lindo Rosa E.Cardoza Alicia Lorenzana Pedro A.Casquero Santiago Gutiérrez 

机构地区：[1]Area of Microbiology,University of León,Campus of Ponferrada,Ponferrada,Spain [2]University Group of Research in Engineering and Sustainable Agriculture,University of León,León,Spain

出　　处：《Journal of Integrative Plant Biology》2020年第7期927-947,共21页植物学报（英文版）

基　　金：We thank Jose Alvarez for his excellent technical assistance.We also thank Prof.Carlos Nicolas from the University of Salamanca(Spain)for providing us with the Arabidopsis thaliana col-o ecotype.This work was supported by the Spanish Ministry of Economy and Competitiveness(MINECO-AGL2015-70671-C2-2-R,MICINN-RTI2018-099600-B-I00 to S.G.);also by the Junta de Castilla y Leon(Spain)(LE251P18).L Undo was granted a fellowship by the University of Leon(Spain).

摘　　要：Trichoderma biocontrol strains establish a complex network of interactions with plants,in which diverse fungal molecules are involved in the recognition of these fungi as nonpathogenic organisms.These molecules act as microbial-associated molecular patterns that trigger plant responses.Previous studies have reported the im-portance of ergosterol produced by Trichoderma spp.for the ability of these fungi to induce plant growth and de-fenses.In addition,squalene,a sterol biosynthetic inter-mediate,seems to play an important role in these inter-actions.Here,we analyzed the effect of different concentrations of ergosterol and squalene on tomato(Solanum lycopersicum)growth and on the transcription level of defense-and growth-related genes.We used an RNA-seq strategy to identify several tomato genesencoding predicted pattern recognition receptor proteins or WRKY transcription factors,both of which are puta-tively involved in the perception and response to ergo-sterol and squalene.Finally,an analysis of Arabidopsis thaliana mutants lacking the genes homologous to these tomato candidates led to the identification of a WRKY40 transcription factor that negatively regulates salicylic acid-related genes and positively regulates ethylene-and jasmonate-related genes in the presence of ergosterol and squalene.

关 键 词：plant PERCEPTION PATTERN 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治] Q943.2[农业科学—植物保护]