The transcription factor GATA10 regulates fertility conversion of a two-line hybrid tms5 mutant rice via the modulation of UbL40 expression^∞  

作　　者：Jing Jin Songtao Gui Qian Li Ying Wang Hongyuan Zhang Zhixuan Zhu Hao Chen Yueyang Sun Yu Zou Xingguo Huang Yi Ding 

机构地区：[1]State Key Laboratory of Hybrid Rice,Department of Genetics,College of Life Sciences,Wuhan University,Wuhan 430072,China [2]Institute of Vegetable,Wuhan Academy of Agricultural Sciences,Wuhan 430072,China [3]Wuhan Wuda Tianyuau Bio-Tech Co.,Ltd.,Wuhan 430070,China

出　　处：《Journal of Integrative Plant Biology》2020年第7期1034-1056,共23页植物学报（英文版）

基　　金：We acknowledge Jinghong Yu(College of Life Sciences,Wuhan University,Wuhan,430072,China)for breeding rice WXS.This research is financially supported by National Natural Science Foundation of China(31471464);the funding from National Program on Key Basic Research Project of China(973 Program:2013CB126900).

摘　　要：The thermosensitive genic male sterile 5(tms5)mutation causes thermosensitive genic male sterility in rice(Oryza sativa)through loss of RNase ZS1 function,which influences ubiquitin fusion ribosomal protein L40(UbL40)messenger RNA levels during male development.Here,we used ATAC-seq,combined with analysis of H3K9ac and H3K4me2,to identify changes in accessible chromatin during fertility conversion of the two-line hybrid rice Wux-iang S(WXS)derived from a mutant tms5 allele.Fur-thermore,RNA-seq and bioinformatic analyses identified specific transcription factors(TFs)in differentially accessible chromatin regions.Among these TFs,only GATA10 targeted UbL40.Osgata10 knockout mutations,which resulted in low expression of UbL40 and a tendency toward male fertility,confirmed that GATA10 regulated fertility conversion via themodulation of UbL40.Meanwhile,GATA10 acted as a medi-ator for interactions with ERF65,which revealed that tran-scriptional regulation is a complex process involving multiple complexes of TFs,namely TF modules.It appears that the ERF141/MADS7/MADS50/MYB modules affect metabolic processes that control anther and pollen development,es-pecially cell wall formation.Our analysis revealed that these modules directly or indirectly affect metabolic pathway-re-lated genes to coordinate plant growth with proper anther development,and furthermore,that GATA10 regulates fer-tility conversion via the modulation of UbL40 expression.

关 键 词：SATIVA FERTILITY STERILE 

分 类 号：S511[农业科学—作物学]