小窝对氧化型低密度脂蛋白诱导小鼠主动脉内皮细胞一氧化氮合酶减弱的作用  

Effect of caveolae on ox-LDL-induced endothelial nitric oxide synthase attenuation in aortic endothelial cells from mice

作　　者：刘欢欢 贾青[2] 张华[2] 赵颖馨[2] 孙尚文[2] 刘振东[2] 柴强[2] Liu Huanhuan;Jia Qing;Zhang Hua;Zhao Yingxin;Sun Shangwen;Liu Zhendong;Chai Qiang(Jinan university,Institute of Basic Medicine,Shandong Academy of Medical Sciences,Jinan 250200,Shandong Province,China)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,250200 [2]山东省医学科学院基础医学研究所

出　　处：《中华老年心脑血管病杂志》2020年第7期688-691,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81470489,81670432,81973139)。

摘　　要：目的探讨氧化型低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)对小鼠主动脉内皮细胞中内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)的影响及可能机制。方法体外培养小鼠主动脉内皮细胞,按照0 mg/L组(对照1组)、25 mg/L组、50 mg/L组、100 mg/L组的ox-LDL浓度梯度分别处理24 h。Western blot检测eNOS、磷酸化eNOS和Caveolin-1(Cav-1)蛋白表达。为了进一步验证ox-LDL是通过影响Cav-1进而减弱eNOS和磷酸化eNOS蛋白表达,设立对照2组和siRNA组,应用Cav-1-mus小干扰RNA(siRNA)敲减Cav-1基因后,观察eNOS和磷酸化eNOS蛋白表达。结果与对照1组比较,25 mg/L组、50 mg/L组、100 mg/L组Cav-1、eNOS和磷酸化eNOS蛋白表达明显降低,差异有统计学意义(P<0.05,P<0.01)。siRNA组Cav-1、eNOS和磷酸化eNOS蛋白表达较对照2组明显降低(0.07±0.02 vs 0.45±0.12,P<0.01;0.34±0.13 vs 0.85±0.38,P<0.05;0.47±0.18 vs 0.86±0.13,P<0.05)。结论ox-LDL降低内皮细胞eNOS蛋白表达可能是通过影响Cav-1蛋白表达而发挥作用。Objective To study the effect of ox-LDL on endothelial nitric oxide synthase(eNOS)attenuation in aortic endothelial cells(AEC)from mice and its mechanism.Methods The AEC from mice were divided into 0 mg/L ox-LDL group(control group 1),25 mg/L ox-LDL group,50 mg/L ox-LDL group and 100 mg/L ox-LDL group after cultured in vitro and treated in different concentrations of ox-LDL for 24 hours.Expressions of eNOS,p-eNOS and caveolin-1(Cav-1)protein were detected by Western blot.The AEC from mice were divided into control group 2 and siRNA group in order to further verify whether ox-LDL attenuates the expressions of eNOS and p-eNOS protein by affecting Cav-1.The expressions of eNOS and p-eNOS protein were detected using the Cav-1 siRNA to knock down the Cav-1 protein.Results The expression levels of Cav-1,eNOS and p-eNOS protein were significantly lower in 25 mg/L ox-LDL group,50 mg/L ox-LDL group and 100 mg/L ox-LDL group than in control group 1(P<0.05,P<0.01)and in siRNA group than in control group 2(0.07±0.02 vs 0.45±0.12,P<0.01;0.34±0.13 vs 0.85±0.38,P<0.05;0.47±0.18 vs 0.86±0.13,P<0.05).Conclusion ox-LDL attenuates the expression of eNOS protein by affecting the expression of Cav-1 protein in AEC from mice.

关 键 词：脂蛋白类 LDL 内皮细胞 动脉粥样硬化 一氧化氮合酶 有被小窝 细胞膜 

分 类 号：R543.5[医药卫生—心血管疾病]