霍山石斛内生真菌DHS-3、DHS-4代谢产物的高效液相研究  

Study on the High Performance Liquid Chromatography of the Metabolites of Endophytic Fungi DHS-3 and DHS-4 Isolated from Dendrobium Huoshanense C.Z.Tang et S.J.Cheng

作　　者：陈传平[1,2] 柯成 陈乃东[2] Chen Chuanping;Ke Cheng;Chen Naidong(l.West Anhui Health Vocational College,Lu'an 237005,China;College of Biotechnology and Pharmaceutical Engineering,West Anhui University,Lu'an 237012,China)

机构地区：[1]皖西卫生职业学院,安徽六安237005 [2]皖西学院生物与制药工程学院,安徽六安237012

出　　处：《黄山学院学报》2020年第3期29-32,共4页Journal of Huangshan University

基　　金：安徽省教育厅高校优秀拔尖人才培育项目(2019gxbjZD76);国家自然科学基金项目(81573536,81274021)。

摘　　要：将已经分离得到的霍山石斛内生真菌DHS-3、DHS-4进行培养,运用高效液相色谱(HPLC)检测技术,对DHS-3、DHS-4的代谢产物进行分析比较。结果表明洗脱峰RT18.353、RT48.536,RT29.442、RT50.358分别为DHS-3、DHS-4两种菌的代谢产物与霍山石斛的共有峰,揭示霍山石斛内生真菌DHS-3、DHS-4可能产生与霍山石斛相同的物质。The endophytic fungi DHS-3 and DHS-4 isolated from dendrobium huoshanense C.Z.Tang et S.J.Cheng were cultured.The metabolites of DHS-3 and DHS-4 were analyzed and compared by HPLC.The results show that the elution peaks RT18.353 and RT48.536,RT29.442 and RT50.358,respectively,were the common peaks of the metabolites of DHS-3 and DHS-4 and dendrobium huoshanense C.Z.Tang et S.J.Cheng,which reveals that the endophytic fungi DHS-3 and DHS-4 could produce the same substances as their host plant dendrobium huoshanense C.Z.Tang et S.J.Cheng.

关 键 词：霍山石斛 内生真菌 代谢产物 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]