PDGFR-β在不同年龄牦牛肺中分布和表达的研究  

Study on the Distribution and Expression of PDGFR-βin the Lungs of Yaks at Different Ages

作　　者：田竹新 张倩[1] 崔燕[1] 何俊峰[1] TIAN Zhuxin;ZHANG Qian;CUI Yan;HE Junfeng(College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070

出　　处：《畜牧兽医学报》2020年第7期1764-1767,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31960687)。

摘　　要：旨在研究血小板源性生长因子受体-β(PDGFR-β)在牦牛肺中的分布和表达,探讨牦牛肺对高原低氧环境的适应机制。采用免疫组织化学、Western-blot技术和qRT-PCR检测方法对新生、1岁、3岁及6岁牦牛肺中PDGFR-β的准确分布位置及表达量进行研究。结果显示:PDGFR-β主要分布在肺内支气管及其分支的上皮细胞、肺动脉血管内皮细胞及管壁平滑肌细胞中,其表达较强;少量分布在支气管管壁平滑肌细胞与肺泡隔细胞中,其表达较弱。在不同年龄段牦牛肺中PDGFR-β蛋白和mRNA也有不同程度的表达。在蛋白水平上,新生组极显著高于其他3组(P<0.01);1岁组、3岁组、6岁组两两相比较,差异性不显著(P>0.05)。在mRNA表达水平上,以新生组的表达最强,与其他3组相比较,差异性极显著(P<0.01);1岁组表达量极显著高于3岁组、6岁组(P<0.01);6岁组显著高于3岁组(P<0.05)。试验表明,PDGFR-β在不同年龄牦牛气道和肺动脉发育及适应性结构的形成过程中均发挥作用,以新生到1岁龄之间的表现最为明显。This study was conducted to investigate the distribution and expression of platelet-derived growth factor receptor-β(PDGFR-β)in yak lungs,and to explore the adaptation mechanism of yak lungs to high altitude hypoxia environment.Immunohistochemistry,Western-blot technology and qRT-PCR were used to study the exact distribution and expression of PDGFR-βin the lungs of newborn,1-year-old,3-year-old,and 6-year-old yak.The results showed that PDGFR-βwas mainly distributed in the bronchial and epithelial cells of the lung,pulmonary vascular endothelial cells,and smooth muscle cells of the bronchial wall,and its expression was strong.A small amount PDGFR-βwas distributed in the smooth muscle cells of the bronchial wall and alveolar septal cells,and its expression was weak.PDGFR-βmRNA and protein were expressed at different levels in the lungs of yak at different ages.In terms of protein level,the newborn group was extremely significantly higher than those of the other 3 groups(P<0.01);in the 1,3,and 6 years old groups,PDGFR-βexpressions were not significantly different when compared with each other(P>0.05).In the mRNA expression level,the expression in the newborn group was the strongest and extremely significantly higher than those of the other 3 groups(P<0.01);PDGFR-βexpression in the 1-year-old group was extremely significantly higher than the 3-year-old group and 6-year-old group(P<0.01);the expression difference was significant between the 3-year-old group and the 6-year-old group(P<0.05).The results indicated that PDGFR-βplayed an important role in the development of airways and pulmonary arteries and in the formation of adaptive structures in yaks at different ages,and this was most obvious in the newborn to 1 year old period.

关 键 词：牦牛 肺 血小板源性生长因子受体-β(PDGFR-β) 

分 类 号：S823.85[农业科学—畜牧学]