清化凉血化瘀方对急性肝衰竭大鼠血清肿瘤坏死因子(TNF-α)、内毒素及肝脏组织病理改变的影响  

Effect of Qinghua Liangxue Huayu Decoction on Serum Tumor Necrosis Factor (TNF-alpha), Endotoxin and Liver Pathological Changes for Rats with Acute Liver Failure

作　　者：林彦[1] 王健[1] 张岚[1] 虞胜[1] LIN Yan;WANG Jian;ZHANG Lan(Municipal Traditional Chinese Medicine Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]上海中医药大学附属市中医医院,上海200071

出　　处：《四川中医》2020年第6期77-80,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：上海市卫生健康委员会中医药科研专项课题(编号:2016LP035)。

摘　　要：目的:观察清化凉血化瘀方对急性肝衰竭大鼠血清肿瘤坏死因子(TNF-α)、内毒素及肝脏组织病理改变的影响。方法:将160只大鼠随机分为:空白组20只、模型组35只、西药组(复方甘草酸苷)35只、中药组(清化凉血化瘀方)35只、中西医结合组(清化凉血化瘀方+复方甘草酸苷)35只,其中西药组和中西医结合组均给予复方甘草酸苷溶液灌胃,中药组给予清化凉血化瘀方灌胃,空白组和模型组均予灭菌蒸馏水灌胃,连续灌胃3天后,除空白组外,其余各组共140只大鼠均分别予D-Ga1800mg/kg、LPS 8μg/kg的剂量腹腔注射,制造急性肝衰竭模型,造模后西药组给予复方甘草酸苷溶液灌胃1次,中药组及中西医结合组给予清化凉血化瘀方灌胃1次。造模24h后处死,腹主动脉采血,测血清肿瘤坏死因子(TNF-α)、内毒素检测。取肝组织病理标本,肉眼观察各组大鼠肝脏组织的外观形态改变,HE染色后在光镜下观察肝组织的病理改变。结果:与模型组相比,各治疗组TNF-α、内毒素都出现了明显下降,中西医结合组低于中药组及西药组(P<0.05),中药组及西药组内毒素组间比较无统计学差异。对于肝组织病理改变:光镜下,模型组可见肝小叶结果紊乱,肝细胞大片坏死,肝窦肝索显示不清,肝组织结构破坏严重,中西医结合组肝小叶结构基本正常,尚能分辨肝窦结构,肝细胞间界限尚清晰,比较肝脏组织病理评分,中西医结合组积分显著低于模型组、西药组、中药组(P<0.05)。结论:清化凉血化瘀方联合复方甘草酸苷可显著降低急性肝衰竭大鼠TNF-α、内毒素水平,减轻肝组织病理学改变,中西医结合治疗具有显著的协同增效作用。Objective:To observe the effect of Qinghua Liangxue Huayu Decoction on serum tumor necrosis factor(TNF-alpha), endotoxin and liver pathological changes for rats with acute liver failure.Methods:Total 160 rats were randomly divided into:20 rats in blank group, 35 rats in model group, 35 rats in western medicine group(Compound Glycyrrhizin), 35 rats in traditional Chinese medicine group(Qinghua Liangxue Huayu Decoction), and 35 rats in the integrated Chinese and western medicine group(Qinghua Liangxue Huayu Decoction+Compound Glycyrrhizin).Except the blank group, a total of 140 rats in each group were intraperitoneally injected with d-ga1800 mg/kg and LPS 8 mg/kg, respectively, to create the acute liver failure model.After the establishment of the model, the western medicine group was given Compound Glycyrrhizin Solution once, and the traditional Chinese medicine group and the combination of traditional Chinese and western medicine group was given Qinghua Liangxue Huayu Decoction once.Blood was collected from abdominal aorta and serum was measured for tumor necrosis factor(TNF-alpha) and endotoxin.Pathological specimens of liver tissues were taken and the appearance and morphology changes of liver tissues in each group were observed by naked eye.After HE staining, pathological changes of liver tissues were observed under light microscope.Results:Compared with the model group, TNF-alpha and endotoxin in these all treatment groups were significantly decreased, and the group of integrated Chinese and western medicine was lower than the group of traditional Chinese medicine and western medicine(P<0.05).There was no statistical difference between the groups of traditional Chinese medicine and western medicine endotoxin.For liver tissue pathological changes:the model group visible results hepatic lobule was disorder, large necrotic liver cells, liver sinus display was not clear, badly damaged liver tissue structure, basic normal lobular architecture with the integrated Chinese and western medicine group, to be able to di

关 键 词：清化凉血化瘀方 急性肝衰竭 氨基半乳糖 脂多糖 

分 类 号：R285.5[医药卫生—中药学]