原发性干燥综合征患者外周血环状RNA hsa_circ_0019413的表达研究  

Expression of hsa_circ_0019413 in the peripheral blood of patients with primary Sjogren's syndrome

作　　者：罗云霞 田进海[2] 陈瑶 赵丽娟 周艳 曹佳 王立斌[2] 竺红[4] Luo Yunxia;Tian Jinhai;Chen Yao;Zhao Lijuan;Zhou Yan;Cao Jia;Wang Libin;Zhu Hong(Second ward of Internal Medicine,Changdu People's Hospital of Tibet Autonomous Region,ChangDu 854000,China;Biochip Center,General Hospital of Ningxia Medical University,Yinchuan 750004,China;School of Clinical Medicine,Ningxia Medical University,Yinchuan 750004,China;Department of Rheumatology,General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]西藏自治区昌都市人民医院内二科,854000 [2]宁夏医科大学总医院生物芯片中心,银川750004 [3]宁夏医科大学临床医学院,银川750004 [4]宁夏医科大学总医院风湿科,银川750004

出　　处：《中华风湿病学杂志》2020年第5期302-305,I0003,共5页Chinese Journal of Rheumatology

基　　金：宁夏回族自治区自然科学基金(2018AAC03145,2020AAC03386)。

摘　　要：目的探讨pSS患者外周血中环状RNA(circRNA)hsa_circ_0019413的表达,以及在pSS疾病发生发展中的作用。方法对4例pSS患者和4名健康对照者的外周血的circRNA变化进行微阵列筛选。以实时定量反转录-PCR(qPCR)验证30例pSS患者和30例对照者外周血中circRNA hsa_circ_0019413的表达差异。建立受试者工作特征曲线(ROC曲线),对外周血中circRNA hsa_circ_0019413的潜在诊断价值进行了分析,并将circRNA hsa_circ_0019413的表达水平与pSS患者临床指标进行相关性分析。结果①通过微阵列分析,在2组之间差异表达437个circRNA[差异倍数(FC)≥2.0,P<0.05],其中上调circRNA 365个,下调circRNA 72个;②通过qPCR验证发现pSS患者circRNA hsa_circ_0019413表达水平高于健康对照组,2组间差异具有统计学意义(P<0.05)。ROC曲线分析表明pSS外周血中circRNA hsa_circ_0019413具有潜在诊断价值[AUC=0.883,95%CI(0.782,0.984),P<0.01];③相关性分析显示circRNA hsa_circ_0019413的表达水平与pSS患者病情评估指数EULAR干燥综合征疾病活动度指数(ESSDAI)、ANA滴度呈正相关(r=0.721,P=0.012;r=0.625,P=0.040),而与IgG、ESR无相关性。结论外周血中的circRNA hsa_circ_0019413可能参与pSS发生发展,可能成为pSS诊断的生物标志物。Objective To investigate the expression of hsa_circ_0019413 in the peripheral blood of patients with primary Sjogren's syndrome(pSS)and its role in the development of pSS disease.Methods Microarray screening of circ ribonucleic acid(circRNA)changes was first performed in the peripheral blood of 4 pSS patients and 4 healthy controls.Real-time quantitative reverse transcription polymerase chain reaction(qPCR)was used to verify the difference in the expression of hsa_circ_0019413 in the peripheral blood of 30 pSS patients and 30 controls.By establishing the receiver operating characteristic(ROC)curve,the potential diagnostic value of hsa_circ_0019413 in peripheral blood was analyzed,and the expression level of hsa_circ_0019413 was correlated with the clinical presentations of patients with pSS.Results①By microarray analysis,437 circRNAs were differentially expressed between the two groups(FC≥2.0,P<0.05),of which 365 were up-regulated and 72 were down-regulated.②The expression level of hsa_circ_0019413 in pSS patients was significantly higher than that in healthy controls by qPCR.The difference between the two groups was statistically significant(P<0.05).It showed that hsa_circ_0019413 in peripheral blood of pSS patients had potential diagnostic value by ROC curve analysis[area under the curve(AUC)=0.883,95%CI(0.782,0.984),P<0.01].③The expression level of hsa_circ_0019413 was positively correlated with the ESSDAI,ANA,titer of the pSS patients by correlation analysis(r=0.721,P=0.012;r=0.625,P=0.040),but not with(immunoglobulin(Ig)G or erythrocyte sedimentation rate(ESR).Conclusion Hsa_circ_0019413 in the peripheral blood may be involved in the development of pSS and may be a biomarker for the diagnosis of pSS.

关 键 词：干燥综合征 环状RNA hsa_circ_0019413 诊断 

分 类 号：R593.22[医药卫生—内科学]