生物合成的谷胱甘肽产品中蛋白残留的检测方法  

Method for Detection of Protein Residues in Biosynthetic Glutathione Products

作　　者：赵士敏 许岗 施细文 彭勇 Zhao Shimin;Xu Gang;Shi Xiwen;Peng Yong(Hu'nan Flag Biotechnology Co.,Ltd.,Hu'nan,410000)

机构地区：[1]湖南福来格生物技术有限公司,湖南410000

出　　处：《当代化工研究》2020年第14期132-134,共3页Modern Chemical Research

摘　　要：开发发酵法或酶法合成的谷胱甘肽产品中微量蛋白残留的检测方法,即膜富集法。并对膜富集法的显色条件进行了优化,对确认的方法进行了验证。方法的检测波长为416nm,显色剂的用量为1.2ml,显色时间为20min。该方法专属性良好,谷胱甘肽样品本身不影响该方法的检测,其不与显色液溴连苯三酚红结合,能够得到准确的检测结果。该方法蛋白含量在0-150μg范围内线性良好(R2=0.996);该方法的检测限(DL)为4.6×10-6;定量限(QL)为13.9×10-6;方法的精密度、重现性良好,同一样品连续测量6次的结果的相对标准偏差(RSD)<3%;加标回收率均在95%-102%之间,准确度较高。膜富集法是检测生物合成产品中微量蛋白残留的好方法。To develop a method for the detection of trace protein residues in glutathione products synthesized by fermentation or enzymatic method.The color development condition of the membrane enrichment method was optimized and the method was verified.Methods the detection wavelength was 416nm,the dosage of chromogenic agent was 1.2ml and the chromogenic time was 20min.The method has good specificity,the glutathione sample itself does not affect the detection of this method,it does not combine with the color solution brominated phenyltriphenyl red,can obtain accurate detection results.The protein content of the method was linear in the range of 0-150μg (R2=0.996).The detection limit (DL) is 4.6×10-6.The quantitative limit (QL) is 13.9×10-6;The accuracy and reproducibility of the method were good.The relative standard deviation (RSD) of the results of 6 consecutive measurements of the same sample was < 3%.The standard recovery rate is 95%-102% with high accuracy.Membrane enrichment is a good method to detect trace protein residues in biosynthetic products.

关 键 词：谷胱甘肽 微量蛋白 膜富集法 溴邻苯三酚红 

分 类 号：Q[生物学]