J亚型禽白血病病毒水平传播的试验  

Experiment on Avian Leukosis Subgroup J Horizontal Transmission

作　　者：张桂枝 龙瑞艳 徐明国 靳双星 ZHANG Gui-zhi;LONG Rui-yan;XU Ming-guo;JIN Shuang-xing(Henan University of Animal Husbandry and Economy,College of Animal Science and Technology,Zhengzhou 450046,China;Henan Poultry Disease Prevention and Control Engineering Technology Research Center,Zhengzhou 450046,China;Shihezi University,College of Animal Science and Technology,Shihezi 832003,China)

机构地区：[1]河南牧业经济学院动物科技学院,河南郑州450046 [2]河南省家禽疾病防控工程技术研究中心,河南郑州450046 [3]石河子大学动物科技学院,新疆石河子832003

出　　处：《中国兽医杂志》2020年第2期1-4,8,共5页Chinese Journal of Veterinary Medicine

基　　金：河南省重大科技专项计划项目(161100510200)。

摘　　要：为探讨J亚型禽白血病病毒(ALV-J)的水平传播情况,选取0日龄60只抗原阴性鸡和20只抗原阳性鸡混合饲养,接触7 d、15 d、25 d、35 d、45 d、55 d、65 d、75 d、90 d后检测阴性鸡的泄殖腔ALV-p27和血清ALV-J抗体,并于65 d采集阴性鸡血浆接种DF-1细胞进行外源性ALV和J亚群分离鉴定。结果显示,阴性鸡与阳性鸡混合饲养15 d,泄殖腔中开始检测到ALV-p27抗原,65 d阳性率最高达50.00%;ELISA和RT-PCR方法检测到细胞培养液中外源性ALV阳性率分别为60.00%和56.67%;RT-PCR和IFA方法检测到细胞培养液中ALV-J抗原阳性率分别为56.67%和63.33%;65 d阴性鸡血清中ALV-J抗体阳性率达63.33%。表明阴性鸡与阳性鸡直接接触混合饲养,通过水平传播被感染外源性ALV-J的几率相当高。In order to investigate the horizontal transmission of avian leukosis subgroup J. 60 antigen-negative and 20 antigen-positive 0-day-old chickens were selected and raised together. 7,15,25,35,45,55,65,75 and 90 days after contact, cloaca ALV-P27 and serum ALV-J antibodies in antigen-negative chickens were detected. The blood samples of antigen-negative chickens were collected at 65 d and inoculated into DF-1 cells for identification of exogenous ALV and ALV-J. The results showed that after mixing antigen-negative and antigen-positive chickens for 15 days, ALV-P27 antigen was found in antigen-negative cloaca and the positive rate of 65 d was up to 50.00%. The ELISA and RT-PCR methods were used to detect exogenous ALV in cell culture medium, and the positive rates were 60.00%and 56.67%, respectively.The RT-PCR and IFA methods were used to detect ALV-J antigen, and positive rates were 56.67% and 63.33%, respectively, ALV-J antibody positive rate reached 63.33% at 65 days. Direct contact mixed feeding of negative chickens and positive chickens resulted in a high probability of being infected with exogenous ALV through horizontal transmission.

关 键 词：禽白血病 J亚型禽白血病病毒 水平传播 

分 类 号：S852.65[农业科学—基础兽医学]