河北省部分地区屠宰猪肝脏戊型肝炎抗原的检测及组织病理学观察  

Study of Detection HEV-AG and Histopathological Observation of Liver of Slaughter Pigs in Some Areas of Hebei Province

作　　者：李睿文[1] 白云 徐瑞涛[1] 白雪峰 LI Rui-wen;BAI Yun;XU Rui-tao;BAI Xue-feng(College of Veterinary Medicine,Agricultural University of Hebei,Baoding 071000,China;Hebei Fengning Manchu Autonomous County Agriculture and Animal Husbandry Bureau Dage Regional Station,Fengning 068350,China)

机构地区：[1]河北农业大学动物医学院,河北保定071000 [2]河北省丰宁满族自治县农牧局大阁区域站,河北丰宁068350

出　　处：《中国兽医杂志》2020年第2期33-35,共3页Chinese Journal of Veterinary Medicine

摘　　要：为探讨河北省部分地区屠宰猪戊型肝炎病毒(HEV)的感染情况,采集了55例屠宰猪肝脏,采用免疫组织化学方法对肝脏样品的戊型肝炎病毒抗原进行检测,同时应用H.E.染色和Mallory三色染色法对其病理形态学变化进行观察。结果显示,55例肝脏样品中有52例呈抗原阳性反应,阳性率为94.55%。光学显微镜观察可见,病毒抗原在肝细胞核、胞浆和胞膜上均有分布,但主要呈现胞浆弥漫型和胞核型。组织病理学观察结果显示,在HEV-抗原免疫组化阳性反应肝脏样品的H.E.染色切片中,均有不同程度的病理变化,主要表现为肝细胞变性散在单个核固缩性坏死,淋巴细胞等炎性细胞浸润,汇管区胆管增生,肝细胞萎缩,并常见有纤维结缔组织增生。Mallory三色染色结果显示,有29.1%的肝脏样品中有纤维结缔组织增生。To investigate hepatitis E virus infection in slaughter pigs in some areas of Hebei province, 55 livers of slaughter pigs were collected in this study. Immunohistochemical staining of liver samples showed that positive material sporadicly distributed in hepatocyte, and the positive rate was 94.55%. Histopathologic observation found sporadic denaturation and karyopynosis of hepatocyte, infiltration of lymphocytes, and hyperplasy of bile canaliculus in portal area. With Mallory staining, fibrous connective tissue proliferation was observed in 29.1% of the liver samples. The results indicate that slaughter pigs are heavily infected with hepatitis E virus in some areas of Hebei.

关 键 词：猪 戊型肝炎 免疫组化 组织病理学 

分 类 号：S852.31[农业科学—基础兽医学]