鹅源鼠伤寒沙门菌的分离鉴定及药敏试验  

Isolation, Identification and Drug Resistance Test of Salmonella Typhimurium from Geese

作　　者：孔铭 高尚 徐磊[1,2] 王彦红 王晓泉[1,2] KONG Ming;GAO Shang;XU Lei;WANG Yan-hong;WANG Xiao-quan(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医杂志》2020年第2期82-84,88,共4页Chinese Journal of Veterinary Medicine

基　　金：江苏高校优势学科建设工程资助项目;现代农业产业技术体系建设专项资金资助(nycytx-41-G07);江苏高校品牌专业建设工程资助项目(PPZY2015B158)。

摘　　要：对临床送检疑似鼠伤寒沙门菌感染的病例进行细菌分离,利用沙门菌所具有的种属特异性的亲膜蛋白基因inv A做PCR鉴定,结合生化鉴定和血清型鉴定,并分析分离菌的耐药性。从4例病死鹅病料中共分离到4株细菌,经inv A PCR鉴定、生化特点和血清型鉴定确认为鼠伤寒沙门菌。药敏试验结果显示,4株分离菌对庆大霉素、诺氟沙星、头孢曲松等药物敏感性较强,但对多黏菌素B、多西环素耐药。其中,有2个分离株表现出多重耐药性。Bacteria were isolated from clinically suspected cases of Salmonella Typhimurium infection, and then identified using PCR for the amplification of the species-specific membranophile protein gene inv A, biochemical test and serotype test. The drug resistance was also analyzed. The results showed that 4 strains were isolated from the diseased geese of 4 farms and identified as Salmonella Typhimurium by PCR amplification, biochemical characteristics and serotype. The drug sensitivity test results showed that 4 isolates were highly sensitive to gentamicin, norfloxacin and ceftriaxone, but resistant to polymyxin B and doxycycline. Two isolates showed multiple drug resistance.

关 键 词：鼠伤寒沙门菌 耐药性 PCR鉴定 

分 类 号：S852.61[农业科学—基础兽医学]