一株致兔结膜炎多杀性巴氏杆菌的分子分型及毒力基因分布  

Molecular Characterisation of Pasteurella Multocida Causing Conjunctivitis in Rabbits

作　　者：王锦祥[1] 孙世坤[1] 陈岩峰[1] 桑雷[1] 陈冬金[1] 谢喜平[1] Wang Jin-xiang;Sun Shi-kun;Chen Yan-feng;Sang Lei;Chen Dong-jin;Xie Xi-ping(Animal Husbandry and Veterinary Research Institute of Fujian Academy of Agricultural Sciences,Fuzhou 350013)

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013

出　　处：《中国养兔》2020年第3期8-10,共3页Chinese Journal of Rabbit Farming

基　　金：福建省公益类科研专项(2019R1026-9);国家兔产业技术体系专项(CARS-43-G-5)。

摘　　要：为明确致兔结膜炎多杀性巴氏杆菌分离株FJPM001的分子分型和毒力基因分布,本试验对分离菌FJPM001进行荚膜分子分型、菌体分子分型和多位点序列分型并对该菌的毒力基因分布进行检测。结果显示,该菌的荚膜为A型,菌体为L3型,确定其血清型为A:L3。多位点序列分型结果显示该菌的序列型为ST12,并携带omp A、omp H、oma87、hgb B、ptf A、tad D和pm HAS 7种毒力基因。本研究分析了致兔结膜炎多杀性巴氏杆菌的分子分型和毒力基因分布,为进一步研究该菌的致病机理奠定基础。In order to determine the molecular typing and virulence gene distribution of the rabbit conjunctivitis polykilling papellosis isolator FJPM001, this experiment conducted capsular molecular typing, bacterial moleculartyping and multi-site sequence typing of the isolator FJPM001, and detected the virulence gene distribution of theisolator FJPM001.The results showed that the capsule of the bacteria was type A, the bacteria was type L3, and theserotype was A:L3. The results of multi-site sequence typing showed that the strain was of sequence type ST12 andcarried seven virulence genes: omp A, omp H, oma87, hgb B, ptf A, tad D and pm HAS. In this study, the molecular typ-ing and virulence gene distribution of pasteurella multikilling in conjunctivitis of rabbits were analyzed, which laid afoundation for further study on the pathogenic mechanism of this bacterium.

关 键 词：兔 多杀性巴氏杆菌 结膜炎 分子分型 毒力基因 

分 类 号：S829.1[农业科学—畜牧学]