miR-1254靶向PAX5调控肝癌的迁移、侵袭与血管形成  

miR-1254 regulates migration,invasion,and vascular formation of liver cancer by targeting PAX5

作　　者：卢旭 杨超 胡元昌 饶建华 成峰 LU Xu;YANG Chao;HU Yuanchang;RAO Jianhua;CHENG Feng(Hepatobiliary Center,the First Affiliated Hospital of Nanjing Medical University,Key Laboratory of Liver Transplantation,Chinese Academy of Medical Sciences,Nanjing 210029,China)

机构地区：[1]南京医科大学第一附属医院肝胆中心中国医学科学院肝移植重点实验室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2020年第5期652-657,668,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81270553,81400650,81470901)。

摘　　要：目的:探讨miR-1254对肝癌细胞生物学行为的影响及其潜在机制。方法:①RT-qPCR检测miR-1254在肝癌和癌旁组织或细胞系中的表达。②构建过表达和敲低miR-1254的肝癌细胞系,Transwell实验与HUVEC体外成管实验研究miR-1254对肝癌细胞迁移、侵袭与血管形成的影响。③Target Scan预测miR-1254的靶基因,RT-qPCR与Western blot测定靶基因的表达,双重萤光素酶报告基因证实miR-1254的靶基因。结果:①miR-1254在肝癌组织和肝癌细胞系中的表达上调(P<0.01);②miR-1254促进肝癌细胞的迁移、侵袭与血管形成;③配对盒基因5(paired box gene 5,PAX5)在肝癌组织和细胞系中的表达下调,PAX5是miR-1254的直接靶标,miR-1254负向调控肝癌中PAX5的表达;④过表达PAX5能够逆转过表达miR-1254对肝癌细胞进展的的促进作用。结论:miR-1254通过靶向PAX5促进肝癌细胞的迁移、侵袭与血管形成,因而它是肝癌的一个潜在的治疗靶标。Objective:This study explores the effects and mechanisms of miR-1254 on liver cancer cells.Methods:①RT-qPCR was used to detect the expression of miR-1254 in liver cancer and adjacent tissues or cell lines.②HUVEC tube-formation assays and transwell assays were used to demonstrate effects of miR-1254 on migration,invasion and vascular formation of liver cancer cells.③Target Scan was used to predict the target gene of miR-1254.RT-qPCR and western blot were used to measure the expression of the target gene.Dual luciferase reporter assay was used to confirm the target gene.Results:①miR-1254 was up-regulated in liver cancer tissues and cell lines.②miR-1254 promoted migration,invasion and vascular formation of liver cancer cells.③The paired box gene 5(PAX5)was down-regulated in liver cancer tissues and miR-1254 negatively regulated the expression of PAX5,which was confirmed to be the direct target of miR-1254.④Over-expressing PAX5 could reverse the promotive effects on HCC cells caused by the overexpresssion of miR-1254.Conclusion:miR-1254 promoted migration,invasion and vascular formation of liver cancer cells through targeting PAX5.So miR-1254 might have the value of target therapy in liver cancer.

关 键 词：肝癌 miR-1254 血管形成 

分 类 号：K735.7[历史地理—历史学]