植物生长调节剂2,4-D对烟草BY-2细胞粘弹性的影响  

Effect of plant growth regulator 2,4-D on viscoelasticity of tobacco BY-2 cells

作　　者：谢杰 周铁安[1,2] 陈宗星 胡家金[1,2] 张煜 XIE Jie;ZHOU Tie'an;CHEN Zongxing;HU Jiajin;ZHANG Yu(Hunan Provincial Engineering Technology Research Center for Cell Mechanics and Function Analysis,Changsha 410128,China;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南省细胞力学与功能分析工程技术研究中心,长沙410128 [2]湖南农业大学生物科学技术学院,长沙410128

出　　处：《植物生理学报》2020年第5期1053-1063,共11页Plant Physiology Journal

基　　金：国家自然科学基金(31870933);湖南省教育厅委托项目(12W004)。

摘　　要：本文基于多次泛音、宽频耗散型石英晶体微天平技术,首次检测了2,4-D作用于模式植物烟草BY-2细胞下细胞壁与细胞骨架粘弹性的动态变化规律。结果表明,0.5 mg·L-12,4-D处理下烟草BY-2细胞壁与细胞骨架均变软,在光学显微镜下观察到细胞内物质运输变快。此外,比较了不同浓度(0.1~10 mg·L-1)2,4-D对BY-2细胞粘弹性的影响,在1 mg·L-12,4-D处理下BY-2细胞的粘弹性变化最明显。Based on the technique of multiple overtones and wide frequency quartz crystal microbalance with dissipating monitoring(QCM-D),the dynamic changes in viscoelasticity of cell wall and cytoskeleton of model plant tobacco BY-2 cells under the treatments of 2,4-D were monitored for the first time.The results showed that 0.5 mg·L-12,4-D softened both the cell wall and cytoskeleton of tobacco BY-2,and the transport of intracellular material became faster as observed under the light microscope.In addition,the effects of different concentrations(0.1–10 mg·L-1)of 2,4-D on the viscoelasticity of BY-2 cells were compared,and the results showed that viscoelastic change was most obvious at the concentration of 1 mg·L-12,4-D.

关 键 词：植物生长调节剂 2 4-D 烟草BY-2细胞 石英晶体微天平 粘弹性 

分 类 号：S482.8[农业科学—农药学] S572[农业科学—植物保护]