HSP27过表达抑制脑心肌炎病毒复制的作用初探  

The Preliminary Study on the Inhibitory Effect of Overexpression of HSP27 in Encephalomyocarditis Virus Replication

作　　者：李倩[1,2] 马瑞仙 许淑娟 陈延宏 李向茸[1] 冯若飞 LI Qian;MA Ruixian;XU Shujuan;CHEN Yanhong;LI Xiangrong;FENG Ruofei(]Key Laboratory of Biotechnology&Bioengineering of State Ethnic Affairs Commission,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China; College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]西北民族大学生物医学研究中心生物工程与技术国家民委重点实验室,兰州730030 [2]西北民族大学生命科学与工程学院,兰州730030

出　　处：《中国细胞生物学学报》2020年第5期812-819,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金项目(批准号:31460665);西北民族大学中央高校基本科研业务费专项资金资助研究生项目(批准号:Yxm2019147);国家民委中青年英才计划[批准号:[2018]98];教育部“创新团队发展计划”项目(批准号:IRT_17R88)资助的课题。

摘　　要：已有研究表明,HSP27在一些病毒的生命周期中发挥着重要作用,但它对于脑心肌炎病毒(encephalomyocarditis virus,EMCV)的调控作用尚不明晰。该研究通过构建人源HSP27的表达质粒pCMV-Myc-HSP27并于HEK293细胞中表达后,接种EMCV检测病毒的复制及相关通路蛋白表达情况。结果显示,过表达HSP27可以抑制EMCV在宿主细胞中的复制,进一步分析表明,HSP27可能是通过正调控IFN-β信号通路中接头分子MAVS、TBK1、IRF3的表达和阻止自噬体与溶酶体的融合实现对EMCV复制的负调控作用。总之,该研究首次表明,HSP27抑制EMCV复制是通过IFN-β信号通路及自噬途径来实现的,这些发现为揭示EMCV感染中宿主因子的调控作用和潜在的抗病毒靶点提供新的见解。HSP27 has been shown to play an important role in the life cycle of some viruses,but its regulatory role in EMCV infection is still unclear.In this study,the plasmid pCMV-Myc-HSP27 of human HSP27 was constructed and expressed in HEK293 cells,then inoculated with EMCV to detect the replication of the virus and the expression of related pathway proteins.The results showed that overexpression of HSP27 could inhibit the replication of EMCV in host cells.Further analysis indicated that HSP27 might negatively regulate the replication of EMCV by positively regulating the expression of adaptor proteins MAVS,TBK1,and IRF3 in the IFN-β signaling pathway and preventing the fusion of autophagosome and lysosome.In summary,this study is the first to show that HSP27 inhibits EMCV replication through the IFN-β signaling pathway and autophagy pathway.These findings provide new insights for revealing the regulatory role of host factors and potential antiviral targets in EMCV infection.

关 键 词：热休克蛋白27 脑心肌炎病毒 细胞自噬 IFN-β信号通路 

分 类 号：S852.65[农业科学—基础兽医学]