芪归银颗粒通过影响脓毒症急性肾损伤大鼠的炎症反应及自噬影响肾功能  

Influence of Qiguiyin Granules on the renal function by affecting inflammatory response and autophagy in acute kidney injury rats with sepsis

作　　者：董磊[1] 段美丽[1] 刘景峰[1] 李甜 庞冉 谢雁鸣[2] 刘清泉[3] DONG Lei;DUAN Mei-li;LIU Jing-feng;LI Tian;PANG Ran;XIE Yan-ming;LIU Qing-quan(Department of Critical Care Medicine,Beijing Friendship Hospital,Capital Madical Universty,Beijing 100050,China;Graduate School of China Academy of Chinese Medical Sciences,Beijing 100700;Beijing Hospital of TCM Affiliated to the Capital Medical University,Beijing 100010)

机构地区：[1]首都医科大学附属北京友谊医院重症医学科,北京100050 [2]中国中医科学院研究生院,北京100700 [3]首都医科大学附属北京中医医院,北京100010

出　　处：《北京中医药》2020年第4期325-330,共6页Beijing Journal of Traditional Chinese Medicine

摘　　要：目的通过观察芪归银颗粒干预下,脓毒症急性肾损伤大鼠的自噬和炎症反应的变化,明确芪归银颗粒对脓毒症急性肾损伤大鼠肾功能的影响与炎症反应和自噬之间的关系。方法应用SD大鼠盲肠结扎穿孔(cecal ligation and puncture,CLP)建立脓毒症模型,诱导大鼠发生急性肾损伤(acute kidney injury,AKI),实验分为3组:假手术组,CLP组,CLP+芪归银组,每组包括6只大鼠,每组分别在监测时间点测量以下指标:①循环指标:血压;②肾功能损伤指标:血清肌酐(Scr),胰岛素样生长因子结合蛋白7(IGFBP7)、组织基质金属蛋白酶抑制剂-2(TIMP2);③炎症指标:肿瘤坏死因子-α(TNF-α)、TOLL样受体4(TLR4)、c-Jun氨基末端激酶(JNK)、丝裂原活化蛋白激酶(MAPK);④自噬指标:LC3-Ⅰ、LC3-Ⅱ。采用SPSS 19.0软件完成统计分析,各组均数需经方差齐性检验为方差齐后,再进行多组均数ANOVA检验。结果CLP组和CLP+芪归银组大鼠血压12 h开始降低,而假手术组无明显变化。CLP组和CLP+芪归银组之间血压差异无统计学意义。CLP组和CLP+芪归银组Scr从12 h开始升高,且与假手术组差异有统计学意义。24 h后CLP+芪归银组比CLP组Scr水平降低。IGFBP-7与TIMP-2乘积CLP组3 h后开始明显升高,而CLP+芪归银组与CLP组比较明显降低。CLP组和CLP+芪归银组TNF-α在3 h、6 h、12 h、24 h和48 h均较0 h升高;CLP组和CLP+芪归银组TNF-α在3 h、6 h、12 h、24 h和48 h均较假手术组升高;3 h、6 h、12 h、24 h和48 h CLP+芪归银组比CLP组降低。CLP组TLR4在6 h、12 h和24 h均较假手术组升高。而CLP+芪归银组在6 h与假手术组比较差异无统计学意义,而与CLP组差异有统计学意义。CLP组MAPK与假手术组比较,6 h、12 h和24 h均有升高,CLP+芪归银组比CLP组降低。CLP组较假手术组LC3-Ⅱ/LC3-Ⅰ升高;在12 h和24 h,CLP+芪归银组较CLP组降低。结论芪归银能够改善脓毒症AKI。脓毒症AKI的变化与炎症反应有关,炎症反应�Objective To determine the influence of Qiguiyin Granules on the renal function and the relationship between the inflammatory response and autophagy in rats with acute kidney injury(AKI)caused by sepsis.Methods The sepsis model was established by using cecal ligation and puncture(CLP)in SD rats to induce AKI.They were divided into three groups:sham operation group,CLP group,CLP+Qiguiyin group with each group 6 rats.Each group was measured with the following indicators at the monitoring time:1.circulating indicators:blood pressure.2.renal function indicators:serum creatinine(sCr),insulin-like growth factor binding protein 7(IGFBP7),tissue matrix metalloproteinase inhibition Agent-2(TIMP2).3.inflammation indicators:tumor necrosis factor-α(TNF-α),TOLL-like receptor 4(TLR4),c-Jun amino terminal kinase(JNK),mitogen-activated protein kinase(MAPK).4.autophagy indicators:LC3-I,LC3-II.Statistical analysis was performed using SPSS19.0 software.The mean of each group was tested by variance homogeneity test,and then multiple sets of mean ANOVA tests were performed.Results The blood pressure of the CLP group and the CLP+Qiguiyin group began to decrease at 12 h,but there was no significant change in the sham operation group.There was no significant difference in blood pressure between the CLP group and the CLP+Qiguiyin group.The serum creatinine in the CLP group and in the CLP+Qiguiyin group increased from 12 h,and were significantly different from the sham operation group.After 24 h,the CLP+Qiguiyin group had lower serum creatinine level than the CLP group.The product of IGFBP-7 and TIMP-2 began to increase significantly after 3 hours in the CLP group,while it in the CLP+Qiguiyin group was significantly lower than that in the CLP group.The levels of TNF-αin CLP group and CLP+Qiguiyin group at 3 h,6 h,12 h,24 h and 48 h were higher than those at 0 h.The levels of TNF-αin the CLP group and CLP+Qiguiyin group were higher than in the sham-operated group at 3 h,6 h,12 h,24 h and 48 h.The levels of TNF-αin the CLP+Qiguiyin gro

关 键 词：脓毒症 急性肾损伤 自噬 芪归银 

分 类 号：R285.5[医药卫生—中药学]