云南省水稻稻瘟病菌无毒基因AVR-Pia变异及水稻抗性基因Pia的有效性  

Variation of avirulence gene AVR-Pia of Magnaporthe oryzae and the effectiveness of rice blast resistance gene Pia in Yunnan Province

作　　者：王群[1] 陆琳[2] 何成兴[1] 伏雪 许明辉[3] 李进斌[1] WANG Qun;LU Lin;HE Chengxing;FU Xue;XU Minghui;LI Jinbin(Agricultural Environment and Resources Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,Yunnan Province,China;Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,Yunnan Province,China;Institute of Biotechnology and Genetic Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205,Yunnan Province,China)

机构地区：[1]云南省农业科学院农业环境资源研究所,昆明650205 [2]云南省农业科学院花卉研究所,昆明650205 [3]云南省农业科学院生物技术与种质资源研究所,昆明650205

出　　处：《植物保护学报》2020年第3期562-571,共10页Journal of Plant Protection

基　　金：国家自然科学基金(31460454);云南省重点项目(2017FA013);云南省人才培养项目(2015HB076)。

摘　　要：为进一步了解田间稻瘟病菌Magnaporthe oryzae群体中AVR-Pia基因的分布及变异,利用水稻单基因系IRBLa-C水稻品种对自云南省13个市(州)采集分离得到的471株稻瘟病菌菌株进行抗性基因Pia有效性测定;利用无毒基因AVR-Pia特异性标记对471株稻瘟病菌菌株进行PCR检测和测序,并分析稻瘟病菌群体中无毒基因AVR-Pia的分布及DNA结构变异;利用有效性结果和PCR检测结果对471株菌株进行反应型划分,筛选鉴定菌株;利用鉴定菌株对云南省112份地方稻种进行Pia基因鉴定。结果表明,在471株稻瘟病菌菌株中,对含有Pia基因的水稻单基因系IRBLa-C表现为抗病和感病的菌株数分别为139株和332株,所占比例分别为29.5%和70.5%;在471株稻瘟病菌菌株中,分别有244株和227株菌株含有无毒基因AVR-Pia和不含有无毒基因AVR-Pia,所占比例分别为51.8%和48.2%,无毒基因AVR-Pia主要为完全缺失变异;在471株稻瘟病菌菌株中,A-和V+反应型菌株数分别为56株和161株,共217株,占总菌株数的46.1%,在13个市(州)稻瘟病菌群体中,A-和V+反应型菌株所占比例差异较大,其中在普洱市、红河哈尼族彝族自治州、昭通市、玉溪市4个市(州)的比例较大,分别为77.8%、57.1%、52.1%和50.0%;在112份云南省地方稻种质资源中,有20份地方稻品种含有抗性基因Pia,主要分布在9个市(州)中。表明云南省13个市(州)绝大部分水稻产区水稻Pia基因已丧抗性,含Pia基因的水稻种质在云南省分布较广。In order to further know distribution and variation of AVR-Pia of Magnaporthe oryzae, total471 isolates of M. oryzae from 13 cities(prefectures) in Yunnan Province of China were used for pathogenicity test to examine the effectiveness of rice blast resistance gene Pia using monogenic line IRBLaC. Genomic DNA of 471 isolates of M. oryzae collected and isolated from different rice-growing-regions in Yunnan Province of China were used for PCR amplification and sequencing to examine the existence and structure variation of AVR-Pia using gene specific PCR markers. The reaction types were classified base on the effectiveness and PCR detection results, and from which to screen the isolates to identify Pia. The Pia was identified among 112 local rice varieties collected from Yunnan Province by using the identification isolates. The results showed that 139 and 332 isolates among the 471 rice blast isolates were avirulent and virulent to the Pia-containing monogenic line IRBLa-C, with the proportion of 29.5% and 70.5%, respectively. Among 471 rice blast isolates, 244 isolates of M. oryzae carry AVRPia and 227 isolates without AVR-Pia, accounting for 51.8% and 48.2%, respectively. The AVR-Pia mainly showed the mutation of complete deletion manner. The 56 and 161 isolates were belonging to the reactive type of A-and V+ respectively, and with the total proportion of 46.1% among 471 detected isolates. The proportion of types A-and V+ were different in 13 cities(prefectures) of Yunnan Province,the proportion of A-and V+ was higher in the isolates population from Puer City, Honghe Hani and Yi Autonomous Prefecture, Zhaotong City, and Yuxi City, the proportion was 77.8%, 57.1%, 52.1% and50.0%, respectively. A total of 20 of 112 local rice varieties holding rice blast resistance gene Pia, and the 20 local rice varieties mainly distributed in nine cities(prefectures) of Yunnan Province. Those results showed that Pia had limited effects in most of rice production areas in 13 cities(prefectures) of Yunnan Province, and the rice germp

关 键 词：水稻 稻瘟病菌 进化 效应子 无毒基因AVR-Pia 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]