Use of testicular sperm in couples with SCSA-defined high sperm DNA fragmentation and failed intracytoplasmic sperm injection using ejaculated sperm  

作　　者：Mohannad Alharbi Fadl Hamouche Simon Phillips Jacques Isaac Kadoch Armand Zini 

机构地区：[1]Division of Urology,Department of Surgery,McGill University,Montreal H4A3J1,Canada [2]Department of Urology,College of Medicine,Qassim University,Qassim 1162,Saudi Arabia [3]OVO Fertility Clinic,Montreal H4P2S4,Canada [4]Department of Obstetrics and Gynecology,University of Montreal,Montreal H3C3J7,Canada

出　　处：《Asian Journal of Andrology》2020年第4期348-353,共6页亚洲男性学杂志（英文版）

摘　　要：Sperm DNA fragmentation(SDF)has been linked with male infertility,and previous studies suggest that SDF can have negative influence on pregnancy outcomes with assisted reproduction.We performed a retrospective review of consecutive couples with a high SDF level that had intracytoplasmic sperm injection(ICSI)using testicular sperm(T-ICSI).We compared the T-ICSI outcomes to that of two control groups:87 couples with failed first ICSI cycle and who had a second ICSI cycle using ejaculated sperm(Ej-ICSI),and 48 consecutive couples with high sperm chromatin structure assay(SCSA)-defined SDF(>15%)that underwent an ICSI cycle using ejaculated sperm after one or more failed ICSI cycles(Ej-ICSI-high SDF).The mean number of oocytes that were retrieved and the total number of embryos were not different among the three groups.The mean number of transferred embryos in the T-ICSI group was higher than the Ej-ICSI group but not significantly different than the Ej-ICSI-high SDF group(1.4,1.2,and 1.3,respectively,P<0.05).Clinical pregnancy rate in the T-ICSI group was not significantly different than the Ej-ICSI and Ej-ICSI-high SDF groups(48.6%,48.2%,and 38.7%,respectively,P>0.05).No significant difference was found in live birth rate when comparing T-ICSI to Ej-ICSI and Ej-ICSI-high SDF groups.The results suggest that pregnancy outcomes and live birth rates with T-ICSI are not significantly superior to Ej-ICSI in patients with an elevated SCSA-defined sperm DNA fragmentation and prior ICSI failure(s).

关 键 词：intracytoplasmic sperm injection male infertility sperm chromatin structure assay sperm DNA fragmentation sperm retrieval testicular sperm 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]