俄罗斯杨树新品种N12玻璃化组培苗恢复培养的研究  

Study on Recovery and Culture of Vitrification Tissue Culture Seedlings of New Russian Poplar Varieties

作　　者：梁立东 李明文 LIANG Lidong(Heihe Academy of Forestry Sciences,Heilongjiang Heihe 164300)

机构地区：[1]黑河市林业科学院,黑龙江黑河164300

出　　处：《林业科技》2020年第4期1-3,共3页Forestry Science & Technology

摘　　要：采用正交表L9(34)进行试验设计,研究了不同培养基、6-BA浓度、KT浓度和琼脂浓度对俄罗斯杨树新品种N12玻璃化组培苗恢复培养的影响。结果表明:不同培养基、6-BA浓度、KT浓度和琼脂浓度对玻璃化组培苗恢复率的影响具有极显著的差异,其作用大小依次为培养基>6-BA浓度>KT浓度>琼脂浓度;促进玻璃化组培苗恢复培养的最佳处理方式为MS培养基、0 mg/L 6-BA、0.5 mg/L KT和9%琼脂,其玻璃化组培苗恢复效果最好。The orthogonal table L9(34)was used for experimental design.The effects of different media,6-BA concentration,KT concentration and agar concentration on the restoration of vitrified tissue culture seedlings of a new Russian poplar variety were studied.The results showed that the effects of different media,6-BA concentration,KT concentration,and agar concentration on the recovery rate of vitrification tissue culture seedlings were extremely significant,and their effects were in the order of medium>6-BA concentration>KT concentration>agar concentration;the best treatment to promote the recovery of vitrification tissue culture seedlings is MS medium,0 mg/L 6-BA,0.5 mg/L KT and 9%agar.The vitrification tissue culture seedling has the best recovery effect.

关 键 词：杨树 玻璃化 组培苗 培养 

分 类 号：S792.119[农业科学—林木遗传育种] S723.132.6[农业科学—林学]