不同产甲烷培养基对微生物群落结构的影响  

Influences of different methanogenic media on the microbial community structure

作　　者：任国领 金锐[3,4] 李蔚 胡敏[2,4] 曲丽娜 侯兆伟 卞立红 REN Guoling;JIN Rui;LI Wei;HU Min;QU Lina;HOU Zhaowei;BIAN Lihong(Heilongjiang Provincial Key Laboratory of Oilfield Applied Chemistry and Technology,Daqing 163712,China;College of Bioengineering,Daqing Normal University,Daqing 163712,China;Exploration and Development Research Institute of Daqing Oilfield Co Ltd,Daqing 163712,China;National Energy Continental Sandstone Old Oilfield Sustainable Development Research and Development Center,Daqing 163712,China)

机构地区：[1]黑龙江省油田应用化学与技术重点实验室,黑龙江大庆163712 [2]大庆师范学院生物工程学院,黑龙江大庆163712 [3]大庆油田有限责任公司勘探开发研究院,黑龙江大庆163712 [4]国家能源陆相砂岩老油田持续开采研发中心,黑龙江大庆163712

出　　处：《大庆石油地质与开发》2020年第4期113-120,共8页Petroleum Geology & Oilfield Development in Daqing

基　　金：黑龙江省青年创新人才计划项目“微生物采油定向激活及‘保护’策略研究”(UNPYSCT-2015116);大庆油田有限责任公司科技项目“化学驱后微生物采油功能菌及激活体系研究”(dqc-2016-hxqg-ky-001);大庆油田有限责任公司科技项目“利用RNA高通量测序技术优选采油功能基因研究”(dqc-2018-hxqg-ky-001)。

摘　　要：为了检测不同培养基富集油藏采出液产甲烷体系中微生物群落结构的差异,应用MiSeq高通量测序技术对不同培养基产甲烷体系中细菌和古菌群落结构进行分析。结果表明:原油、GD+S、GD+N和K培养基富集细菌分别检测到181、259、280和305个OTU数,古菌分别检测到72、157、153和132个OTU数。原油和K培养基富集得到的优势菌属较为相似,优势细菌为厌氧杆菌属(Anaerobaculum sp.)和未命名细菌(norank_c_W5);优势古菌为未命名古菌(unclassified_k_norank)、产甲烷囊菌属(Methanoculleus sp.)和未命名TMEG(norank_f_Terrestrial_Miscellaneous_Gp_TMEG_),属于未知功能古菌和氢营养型产甲烷古菌;GD+S和GD+N培养基富集得到的优势菌属较为相似,优势细菌为硫杆状菌属(Tepidiphilus sp.)和厌氧杆菌属(Anaerobaculum sp.);优势古菌为未命名古菌(unclassified_k_norank)和产甲烷囊菌属(Methanoculleus sp.),属于未知功能古菌和氢营养型产甲烷古菌。综合产气量和甲烷含量以及优势细菌与古菌的适配性考虑,最优产甲烷培养基为GD+N培养基。In order to detect the differences of the microbial community structures in the methanogenic system of reservior production liquid enriched by different media,MiSeq high-throughput sequencing technology was used to analyze the community structures of the bacteria and archaea in different methanogenic media.The results show that 181,259,280 and 305 OTU both of which wewe enriched were detected in the bacteria and 72,157,153 and 132 OTU(Operational Taxonomic Units)were detected in the archaea,both of which were enriched in the crude oil,GD+S,GD+N and K media.The dominant genera enriched by the crude oil and K media are similar,the dominant bacteria are Anaerobaculum sp.and unclassified bacteria(norank_c_W5);and the dominant archaea are unclassified archaea(unclassified_k_norank),Methanoculleus sp.and unclassified TMEG(norank_f_Terrestrial_Miscellaneous_Gp_TMEG_),which are mainly unknown functional archaea and hydrogenotrophic methanogenic archaea.The dominant genera enriched by GD+S and GD+N media are similar,the dominant bacteria are Tepidiphilus sp.and Anaerobaculum sp.,and the dominant archaea are unclassified archaea(unclassifie d_k_norank)and Methanoculleus sp.,which are mainly unknown functional archaea and hydrogenotrophic methanogenic archaea.Considering the gas production,methane content and adaptability of the dominant bacteria and archaea,the optimal methanogenic medium is GD+N one.

关 键 词：微生物群落结构 甲烷 16S rRNA 高通量测序 原油降解 

分 类 号：TE357.46[石油与天然气工程—油气田开发工程]