日本血吸虫融合表达抗原rSjELAV-like 1和rSjELAV-like 2的免疫保护研究  

STUDY ON IMMUNOPROTECTION OF SCHISTOSOMA JAPONICUM RSJELAV-LIKE 1 AND RSJELAV-LIKE 2

作　　者：秦芳林 李肖纯 程贵凤 吴洛滨 任雨琪 金亚美[1] QIN Fang-lin;LI Xiao-chun;CHENG Gui-feng;WU Luo-bin;REN Yu-qi;JIN Ya-mei(Key Laboratory of Animal Parasitology of the Ministry of Agriculture,Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;College of Life and Environment Science,Shanghai Normal University,Shanghai 200234,China;College of Animal Science and Technology,Shanxi Agricultural University,Shanxi 030800,China)

机构地区：[1]中国农业科学院上海兽医研究所农业农村部动物寄生虫学重点开放实验室,上海200241 [2]上海师范大学生命与环境科学学院,上海200234 [3]山西农业大学动物科技学院,山西030800

出　　处：《中国动物传染病学报》2020年第4期65-70,共6页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金(31672245);上海市自然科学基金(162R1444100)。

摘　　要：本研究通过克隆、表达、纯化日本血吸虫胚胎致死异常视觉基因1和2(SjELAV-like 1和SjELAV-like 2),并将获得的纯化蛋白rSjELAV-like 1和rSjELAV-like 2与206佐剂混合免疫BALB/c小鼠,评估rSjELAV-like 1和rSjELAV-like 2的免疫保护效果。结果显示:rSjELAV-like 1和rSjELAV-like 2在减轻宿主虫荷和肝卵方面均有一定的免疫保护作用,rSjELAV-like 2免疫可明显影响虫卵孵化能力;在第1次免疫后rSjELAV-like 2 的特异性IgG抗体水平与对照相比,呈显著升高,具有统计学意义。本研究结果表明,rSjELAV-like 2 比rSjELAV-like 1更适合作为疫苗候选分子,为日本血吸虫疫苗的研发奠定了物质基础。To evaluate the immunoprotection of embryo lethal abnormality visual genes 1 and 2 (SjELAV-like 1 and SjELAV-like 2) of Schistosoma japonicum,these genes were cloned,expressed and the recombinant proteins rSjELAV-like 1 and rSjELAV-like 2 were purified to evaluate their immunoprotection.The purified proteins were emulsified with 206 adjuvant and injected into mice via subcutaneous route.The results showed that both recombinant proteins had a certain effect on reducing worms and eggs burdens.rSjELAV-like 2 immunization effects eggs hatching.Moreover,the immunized mice developed specific IgG antibodies to rSjELAV-like 2 post the first immunization as determined in ELISA.This study evaluated the immunoprotective effects of rSjELAV-like 1 and rSjELAV-like 2,and rSjELAV-like 2 was more suitable as a vaccine candidate for S.japonicum.

关 键 词：日本血吸虫 rSjELAV-like 1 rSjELAV-like 2 免疫保护 

分 类 号：S852.735[农业科学—基础兽医学]