抗虫转基因甘蔗的培育及其抗性丧失的防控策略  

Breeding of Transgenic Insect-resistant Sugarcane and Strategies for Preventing the Its Resistance to Insects from Loss

作　　者：冯翠莲[1] 张树珍[1] FENG Cui-lian;ZHANG Shu-zhen(Institute of Tropical Bioscience and Biotechnology,CATAS,Sugarcane Research Center,Ministry of Agriculture Key Biotechnology Laboratory for Tropical Crops,Haikou 571101)

机构地区：[1]中国热带农业科学院热带生物技术研究所甘蔗研究中心农业部热带作物生物技术重点开放实验室,海口571101

出　　处：《生物技术通报》2020年第7期209-219,共11页Biotechnology Bulletin

基　　金：中国热带农业科学院基本科研业务费专项(630052020005);海南省青年科技英才创新计划项目(HAST201626)。

摘　　要：由于甘蔗存在遗传背景复杂和抗虫种质资源缺乏的问题,造成甘蔗常规抗虫育种远远落后于其他作物的现状。基因工程为抗虫甘蔗的育种提供了一条崭新的途径。经过资料收集和整理并结合作者所在研究团队的研究成果,综述了近年来国内外抗虫转基因甘蔗的培育现状。首先介绍了甘蔗转基因遗传转化系统及其转基因的遗传稳定性研究的新发展,然后重点介绍了国内外在抗虫转基因甘蔗研究方面取得的突破性研究进展,尤其在通过Bt基因的改造和抗虫基因聚合等策略来防止转基因甘蔗的抗虫性下降甚至丧失等方面进行了详细的阐述,旨在为今后抗虫转基因甘蔗的育种工作提供参考。Due to the complexity of the sugarcane genome and the lack of insect-resistant germplasm resources,the conventional insectresistant breeding of sugarcane is far behind other crops.Currently,genetic engineering provides a novel approach for insect-resistant sugarcane breeding.The recent development status of transgenic insect-resistant sugarcane was reviewed via literature collection and sorting combined with the research achievements in our team.First,the new development of transgenic sugarcane transformation system and studying genetic stability of transgenes were introduced,and then the breaks in studying transgenic insect-resistant sugarcane were focused.In particular,the strategies for preventing the insect resistance of transgenic sugarcane from decreasing or even losing by modifying Bt genes and utilizing gene stacking were introduced in detail,which will provide reference for insect-resistant transgenic sugarcane breeding in the future.

关 键 词：甘蔗 抗虫 Bt基因改造 基因聚合 多基因转化 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]