体液免疫功能、血清sIL-6R水平和血沉的检测在儿童CA-MPP病情变化和疗效评估中的应用  

Application of humoral immune function,serum sIL-6R level and erythrocyte sedimentation rate in the evaluation of changes in CA-MPP condition and curative effect in children

作　　者：陈建州 吴云刚 张辉 CHEN Jianzhou;WU Yungang;ZHANG Hui(Department of Pediatrics,Nanshi Hospital,Nanyang,Henan,China,473000)

机构地区：[1]南阳南石医院儿科,河南南阳473000

出　　处：《分子诊断与治疗杂志》2020年第7期878-881,共4页Journal of Molecular Diagnostics and Therapy

基　　金：河南省医学科技攻关计划项目(2018073215)。

摘　　要：目的研究体液免疫功能、血清sIL-6R水平和血沉的检测在儿童CA-MPP病情变化和疗效评估中的应用.方法选取2018年1月至2019年6月本院收治的90例CA-MPP儿童作为研究对象,根据CPIS评分将研究对象分为两组,CPIS≥6分为CA-MPP重症组(n=46),CPIS<6分为CA-MPP普通组(n=44);另选取同期体检的健康儿童45例设为对照组.散射比浊法测定各组IgA、IgG、IgM水平以及C3、C4水平;ELISA法测定IL-6、sIL-6R以及TNF-α水平;全自动生化分析仪测定ESR水平.结果CA-MPP重症组、普通组患儿血清IgA、IgG、IgM、C3、C4水平以及IL-6、sIL-6R、TNF-α、ESR水平均高于对照组,差异有统计学意义(P<0.05),CA-MPP重症组血清上述指标水平高于CA-MPP普通组,差异有统计学意义(P<0.05).Pearson相关性分析发现,体液免疫功能、血清sIL-6R和血沉的水平均与儿童CA-MPP病情严重程度呈正相关(P<0.05).治疗后重症与普通组CA-MPP儿童血清IgA、IgG、IgM、C3、C4水平以及IL-6、sIL-6R、TNF-α、ESR水平均低于治疗前,差异有统计学意义(P<0.05).结论体液免疫功能、血清sIL-6R水平和血沉的检测能反映儿童CA-MPP病情变化情况,密切监测CA-MPP患儿的上述指标水平对于患儿的病情和疗效评估具有重要意义.Objective To study the application of humoral immune function,serum sI-6R level and erythrocyte sedimentation rate in the assessment of the changes and therapeutic effects of CA-MPP in children.Methods Ninety-eight children with CA-MPP admitted to our hospital from January 2018 to June2019 were enrolled.The subjects were divided into two groups according to the CPIS score.CPIS≥6 was divided into CA-MPP severe group(n=46),CPIS<6 is divided into CA-MPP common group(n=44).a Aother45 healthy children who were selected for physical examination in our hospital were set as the control group.The levels of IgA,IgG,IgM and C3 and C4 in each group were determined by nephelometry.The levels of IL-6,sIL-6R and TNF-αwere determined by ELISA.The ESR level was determined by automatic biochemical analyzer.Results The serum levels of IgA,IgG,IgM,C3,C4,IL-6,sIL-6R,TNF-αand ESR in the severe group and the common group of CA-MPP were higher than those in the control group(P<0.05),and the serum levels of the severe group of CA-MPP were higher than those in the common group of CA-MPP(P<0.05).Pearson correlation analysis showed that humoral immune function,serum sIL-6R and ESR levels were positively correlated with the severity of CA-MPP in children(P<0.05).The serum levels of IgA,IgG,IgM,C3,C4,IL-6,sIL-6R,TNF-αand ESR in the severe group and the common group were lower than those before treatment(P<0.05).Conclusion The detection of humoral immune function,serum sIL-6R level and erythrocyte sedimentation rate can reflect the changes of CA-MPP in children,and closely monitoring the levels of above-mentioned indicators in children with CA-MPP is of great significance for the assessment of the condition and efficacy of children.

关 键 词：体液免疫功能 SIL-6R 血沉 儿童CA-MPP 病情 评估 

分 类 号：R725.6[医药卫生—儿科]