HPLC-MS/MS法测定烟叶中9种B族维生素  

Determination of nine B vitamins in tobacco leaves by high performance liquid chromatographytandem mass spectrometry

作　　者：刘萍萍[1] 申娟娟 卢紫舒 李泽锋[1] 张慧[1] 徐国云[1] 王晨[1] 金立锋[1] 曹培健[1] 周会娜[1] 陈千思[1] LIU Pingping;SHEN Juanjuan;LU Zishu;LI Zefeng;ZHANG Hui;XU Guoyun;WANG Chen;JIN Lifeng;CAO Peijian;ZHOU Huina;CHEN Qiansi(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;College of Chemistry and Molecular Engineering,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]郑州大学化学与分子工程学院,郑州450001

出　　处：《烟草科技》2020年第7期33-39,共7页Tobacco Science & Technology

基　　金：国家烟草专卖局资助项目“烟草基因功能元件全景图构建及关键基因挖掘与调控节点研究”[110201601033(JY-07)];国家烟草专卖局资助项目“典型香型烟草品质形成的代谢特征研究”[110201401012(JY-12)]。

摘　　要：为准确测定烟叶样品中水溶性B族维生素的含量(质量分数),通过优化色谱流动相pH、柱温、提取剂及其体积等参数,建立了同时测定烟叶中9种B族维生素的高效液相色谱-三重四极杆质谱(HPLC-MS/MS)方法,并采用该方法对3个不同产区的烟叶样品进行了测定。结果表明:①在线性范围内,9种B族维生素工作曲线的线性关系良好,决定系数(R^2)为0.9946~0.9999。②该方法的回收率为91.00%~108.79%,日内和日间精密度(RSD)为1.25%~9.73%,9种B族维生素的检出限为0.01~0.50 ng/mL。③3个产区烟叶中B族维生素含量有一定差异,其中河南襄城县产区烟叶中VB2、VB7、VB9的含量较高,贵州贵阳市产区烟叶中VB3的含量较高。该方法操作简便,精密度较好,灵敏度较高,适用于烟叶中多种B族维生素的同时分析。A liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for simultaneous quantification of nine water-soluble B vitamins in tobacco was established by adopting multi-reaction detection mode(MRM).The parameters,such as pH value of mobile phase,column temperature,extraction solvent and volume,were optimized.Tobacco leaf samples collected from three growing areas were analyzed with the developed method.The results showed that:1)There was a good linear relationship for the nine B vitamins with the determination coefficient(R^2)of 0.9946-0.9999.2)The recoveries of the nine B vitamins ranged from 91.00%to 108.79%.The intra-and inter-day relative standard deviations(RSDs)were 1.25%-9.73%.The limits of quantification(LOQ)for all the analytes were from 0.01 to 0.50 ng/mL.3)The contents of B vitamins differed among the leaves from the three growing areas.The contents of VB2,VB7 and VB9 in tobacco leaves from Xiangxian County in Henan were higher than those of the others,and the VB3 content in tobacco leaves from Guiyang City in Guizhou was higher.This method is simple in operation and features good repeatability and high sensitivity,it can be used for the simultaneous analysis of the nine B vitamins.

关 键 词：烟叶 B族维生素 高效液相色谱-串联质谱(HPLC-MS/MS) 

分 类 号：TS411.1[农业科学—烟草工业]