基于响应面法优化兰州百合芽增殖培养基  

Optimization of Lanzhou Lily Bud Proliferation Medium Based on Response Surface Method

作　　者：裴怀弟[1] 林玉红[1] 李琦 李淑洁[1] 张艳萍[1] PEI Huaidi;LIN Yuhong;LI Qi;LI Shujie;ZHANG Yanping(Institute of Biotechnology,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China)

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730070

出　　处：《甘肃农业科技》2020年第7期7-12,共6页Gansu Agricultural Science and Technology

基　　金：国家自然科学基金地区基金项目(31960599);甘肃省科技重大专项(18ZD2NA010);甘肃省农业科学院中青年基金项目(2017GAAS94);甘肃省农业科学院科技支撑项目(2017GAAS36);甘肃省农业科学院重点研发计划(2019GAAS019)。

摘　　要：以兰州百合鳞片为外植体获得的小鳞芽为材料,利用响应面法的Box-Behnken设计方法,对百合组培芽增殖进行试验优化。结果从响应面法优化模型得出的芽增殖最佳培养基为MS+1.25 mg/L 6-BA+0.2 mg/L NAA,在此条件下,增殖系数达3.82。The buds of small scales obtained from Lanzhou Lily’s scales as explants were used as materials,the Box-Behnken design method of response surface method was used to optimize the bud proliferation of lily tissue culture.The results showed that the optimal medium for bud proliferation obtained from the response surface method was MS+1.25 mg/L 6-ba+0.2 mg/L NAA.Under this condition,the bud multiplication coefficient reached 3.82.

关 键 词：响应面法 兰州百合 芽增殖 增殖系数 培养基 

分 类 号：S644.1[农业科学—蔬菜学]