RNA沉默转基因技术在植物抗病虫害研究中的作用  

The plant resistances to viruses and pests mediated by transgenic RNA silencing technology

作　　者：杨美玲[1] 李琦 陈佳琦 张晓明 YANG Meiling;LI Qi;CHEN Jiaqi;ZHANG Xiaoming(State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]中国科学院动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京100101

出　　处：《植物保护》2020年第4期1-6,共6页Plant Protection

基　　金：转基因生物新品种培育科技重大专项(2016ZX08010001);国家重点研发计划(2017YFD0200406,2017YFD0200904)。

摘　　要：RNA沉默技术由于其简便、高效及高特异性在各种模式和非模式生物系统中得到了广泛的应用。植物转基因技术是将植物、动物或微生物中分离到的目的基因,通过各种手段整合到植物基因组上,使其稳定遗传并赋予植物新的优良性状的生物技术。RNA沉默在植物转基因工程领域的研究十分广泛,目前已经成功研究培育出许多抗病毒、抗虫转基因植物,为农业病虫害的防控提供了新的思路和方法。本文重点综述了近年来植物转基因介导RNA沉默在抗虫与抗病毒研究方面的进展,加深人们对转基因植物抗虫与抗病毒研究的认识。RNA silencing,a powerful technique that permits tissue-specific,simple and efficient gene knockdown,has been widely applied in model and non-model organisms.With transgenic technology,recombinant DNA fragments from plants,animals,or microbes are transformed into plants to generate transgenic plants with better characteristics.RNA silencing has been widely used to create transgenic plants that show higher resistance to viruses or pests relative to non-transformed ones.In this paper,we reviewed the recent advancements in the functions of transgenic RNA silencing in plant resistances to viruses and pests.It will improve our understanding of the function of transgenic technology in plant resistances to viruses and pests.

关 键 词：RNA沉默 转基因技术 植物抗虫 植物抗病毒 

分 类 号：S43[农业科学—农业昆虫与害虫防治] Q943.2[农业科学—植物保护]