厚皮香枯梢病病原菌鉴定  

Identification of pathogen causing dieback on Ternstroemia gymnanthera

作　　者：孙荣华 徐颖 王章训 路广亮 许能春 黄国培 SUN Ronghua;XU Ying;WANG Zhangxun;LU Guangliang;XU Nengchun;HUANG Guopei(Shanghai Academy of Landscape Architecture Science and Planning,Shanghai 200232,China;Shanghai Engineering Research Center of Landscaping on Challenging Urban Site,Shanghai 200232,China;Shanghai Gardens Group Co.,Ltd,Shanghai 200335,China;Zhejiang Agriculture and Forestry University,Hangzhou 311300,China)

机构地区：[1]上海市园林科学规划研究院,上海200232 [2]上海城市困难立地绿化工程技术研究中心,上海200232 [3]上海园林(集团)有限公司,上海200335 [4]浙江农林大学,杭州311300

出　　处：《植物保护》2020年第4期185-188,共4页Plant Protection

基　　金：上海市园林科学规划研究院青年人才发展基金(2018-1-7)。

摘　　要：厚皮香枯梢病在上海地区发生严重。本研究对上海地区的厚皮香枯梢病进行了病原菌的分离与致病性鉴定,得到其病原菌菌株2017SHTg。根据病原菌的形态特征将其鉴定为小新壳梭孢Neofusicoccum parvum。扩增病原菌2017SHTg的rDNA-ITS序列和翻译延伸因子EF-1α基因序列,进行BLAST序列比对与小新壳梭孢相似性分别为99%和100%;基于rDNA-ITS序列构建系统发育进化树,其在系统发育树上与小新壳梭孢的类群处于同一分支,此结果与形态学鉴定结果一致。Dieback is a widespread disease on Ternstroemia gymnanthera in Shanghai.Isolate 2017SHTg was proved as causal pathogen through isolation and pathogenicity test.Morphological characters of 2017SHTg were in agreement with Neofusicoccum parvum.Sequence analysis on rDNA-ITS and EF-1αof 2017SHTg showed 99%and 100%similarity to that of N.parvum,and phylogenetic analysis also showed 2017SHTg is attributed to the same branch with N.parvum in phylogenetic tree.These results showed that the causal pathogen causing dieback on T.gymnanthera in Shanghai is N.parvum.

关 键 词：厚皮香 枯梢病 小新壳梭孢 

分 类 号：S432.44[农业科学—植物病理学]