A型口蹄疫病毒抗体化学发光免疫分析检测方法的建立  

Establishment of Chemiluminescent Immunoassay for Detection of Type A FMDV Antibody

作　　者：孙雨[1] 宋晓晖[1] 王睿男[1] 李秀梅 肖颖[3] 任雪建 李晓霞 魏巍[1] 杨林[1] 王传彬[1] SUN Yu;SONG Xiao-hui;WANG Rui-nan;LI Xiu-mei;XIAO Ying;REN Xue-jian;LI Xiao-xia;WEI Wei;YANG Lin;WANG Chuan-bin(China Animal Disease Control Center,Beijing,102600,China;Luoyang Modern Biotechnology Research Institute Co.,Ltd.,Luoyang,Henan,471000,China;Chongqing Animal Disease Control Center,Chongqing 400000,China)

机构地区：[1]中国动物疫病预防控制中心,北京102600 [2]洛阳现代生物技术研究院有限公司,河南洛阳471000 [3]重庆市动物疫病预防控制中心,重庆400000

出　　处：《动物医学进展》2020年第8期29-36,共8页Progress In Veterinary Medicine

基　　金：国家重点计划研发项目(2016YFD0500901);现代农业人才支撑计划(2130106-024);跨境动物疫病防控机制建设(2130114-FK);农业技术实验示范与服务支持(净化)(2130106-039);现代农业产业技术体系建设北京市创新团队(BAIC02-2020)。

摘　　要：为建立动物血清中的A型口蹄疫病毒抗体化学发光免疫分析检测方法,通过获得的A型口蹄疫病毒VP1融合蛋白与相关单克隆抗体建立了A型口蹄疫病毒抗体竞争化学发光检测试剂盒。该方法能够特异性的定量检测猪、牛、羊血清中的A型口蹄疫病毒VP1蛋白抗体,敏感性高,特异性强,对其他相关的偶蹄类动物病原无交叉反应,批内变异系数小于10%,批间变异系数小于15%,具有良好的重复性。对该方法的特异性指标和敏感性指标进行了验证,同国产的A型口蹄疫抗体检测试剂盒进行了比较,阳性样品符合率为92.63%,阴性样品符合率为93.98%,总符合率为93.17%。建立的A型口蹄疫病毒抗体化学发光免疫分析检测方法,特异性高,敏感性强,操作简单、快速,具有较高的应用推广价值。To establish a chemiluminescence immunoassay method for detecting type A FMDV antibody in animal serum,a competitive chemiluminescence assay kit for type A FMDV antibody was established by combining the obtained type A FMDV VP1 fusion protein with relevant monoclonal antibodies.This method can specifically and quantitatively detect the antibody of type A FMDV in the sera of pigs,cattle and sheep.This method has high sensitivity and specificity,no cross-reaction to other related pathogens.The coefficients of variation within and between groups were all less than 10%,and it has good repeatability.Compared with the domestic antibody detection kit for type A FMDV,the coincidence rate of positive samples was 92.63%,the coincidence rate of negative samples was 93.98%,and the total coincidence rate was 93.17%.The chemiluminescence immunoassay method for type A FMDV antibody established in this study is characterized by high specificity,high sensitivity,simple operation,high application and promotion value.

关 键 词：A型口蹄疫病毒 化学发光 免疫分析 检测方法 

分 类 号：S851.33[农业科学—预防兽医学] S852.659.6[农业科学—兽医学]