复方紫草油治疗唇炎机制的网络药理学研究  

Network Pharmacology Study on the Mechanism of Compound Oleum Lithospermi in the Treatment of Cheilitis

作　　者：任雪雯 李元文[1] 蔡玲玲[1] 肖飞 陈艳明[3] REN Xuewen;LI Yuanwen;CAI Lingling;XIAO Fei;CHEN Yanming(Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Jianmin Group Children′s Medicine Research Institute,Wuhan 430052,China;School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China)

机构地区：[1]北京中医药大学东方医院,北京100078 [2]健民集团儿童药物研究院,武汉430052 [3]天津大学药物科学与技术学院,天津300072

出　　处：《世界中医药》2020年第14期2036-2042,共7页World Chinese Medicine

基　　金：北京市自然科学基金面上项目(7192116)——基于miR-145/Sema3A通路探究青石止痒软膏软膏对炎症性皮肤病的止痒机制;北京市中医管理局课题——中医药继续教育导航工程-继教专委会建设及精品课程制作;北京中医药大学横向科研项目(HX201706)——复方紫草油治疗慢性湿疹(血虚风燥证)的临床及动物实验研究。

摘　　要：目的:利用网络药理学的技术探索复方紫草油治疗唇炎的机制,为后续实验研究及扩大复方紫草油的适应证提供理论基础。方法:依托中药系统药理学平台(TCMSP)、中药综合数据库(TCMID)筛选复方紫草油的有效化合物和作用靶点;通过检索人类基因信息数据库(GeneCards)及人类孟德尔遗传研究平台(OMIM)选取唇炎的疾病靶点,并利用R语言取疾病与药物靶点交集;应用Cytoscape软件构建药物-化合物-靶点-疾病互作网络;运用STRING平台搭建蛋白质间相互作用网络(PPI);使用Bioconductor生物信息软件包进行GO功能富集和KEGG通路分析。结果:筛选得103个化合物和相应115个靶点,其中有21个与疾病相关。GO功能富集分析得到22个条目,KEGG通路富集分析筛选得到91条信号通路。结论:复方紫草油治疗唇炎具有生物学支撑。不同的蛋白存在协同作用,其中IL-6、STAT3和TNF等靶点起着关键作用。生物过程主要涉及细胞因子受体结合、金属内肽酶活性等,并主要富集在AGE-RAGE、FssAs和TNF等多条信号通路上,发挥抗炎及调控细胞增殖和凋亡等作用。Objective:To provide a theoretical basis for the follow-up experimental research and expanding the indication of compound oleum lithospermi by using the technology of network pharmacology to explore the mechanism of compound oleum lithospermi in the treatment of cheilitis,Methods:Based on TCMSP(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform)and TCMID(Traditional Chinese Medicine Integrative Database)to screen the effective compounds and targets of Compound Oleum Lithospermi.GeneCards(The Human Gene Database)and OMIM(Online Mendelian Inheritance in Man)was used to select the disease targets of Cheilitis.R language was used to get the intersection of disease and drug target.Cytoscape software was used to build drug-compound-target-disease interaction network;STRING platform was used to build protein-protein interaction network(PPI);Bioconductor biological information software package was used for GO function enrichment and KEGG pathway analysis.Results:A total of 103 compounds and 115 corresponding targets were screened,of which 21 were related to diseases.GO functional enrichment analysis yielded 22 entries,and KEGG pathway enrichment analysis screening yielded 91 signal pathways.Conclusion:Compound oleum lithospermi has biological support in the treatment of cheilitis.Different proteins have synergistic effects,among which IL-6、STAT3 and TNF etc.play a key role.The biological process mainly involves cytokine receptor binding,metalloendopeptidase activity,etc.These targets are mainly concentrated in multiple signaling pathways such as AGE-RAGE、FssAs and TNF,which play key roles of anti-inflammatory and regulation of cell proliferation and apoptosis,etc.

关 键 词：网络药理学 复方紫草油 唇炎 抗炎机制 信号通路 

分 类 号：R242[医药卫生—中医临床基础] R285[医药卫生—中医学]