杉木组培苗生根质量优化研究  

Study on rooting condition optimization of tissue culture seedling of Chinese fir

作　　者：蒋凡 买凯乐[1] JIANG Fan;MAI Kai-le(Guangxi Eco-engineering Vocational and Technical College,Liuzhou 545004,Guangxi,China)

机构地区：[1]广西生态工程职业技术学院,广西柳州545004

出　　处：《湖北农业科学》2020年第11期81-83,88,共4页Hubei Agricultural Sciences

基　　金：广西高校中青年教师科研基础能力提升项目(KY2016YB692)。

摘　　要：从两个方面优化杉木[Cunninghamia lanceolata(Lamb.)Hook.]组培苗生根质量,一是在增殖阶段对其进行高浓度和低浓度激素培养基交替处理以优化组培芽苗的质量,从而达到优化杉木组培苗生根质量;二是在生根阶段改变光源,以优化杉木组培苗生根质量。结果表明,高浓度激素和低浓度激素交替处理后的杉木增殖芽苗,具有增殖率高、生长快、节间距适中、木质化程度较高等表观性状,且这些性状随着继代次数的增加表现比较稳定,增殖芽接种到生根培养基后,生根率最高达到86.5%,生根数量最高达到2.7条。LED复合光源红蓝光配比为2∶1时生根率最高,达到86.7%;在红蓝光配比为4∶1时生根数量最高,达到2.8条。The rooting quality of Chinese fir[Cunninghamia lanceolata(Lamb.)Hook.]tissue culture seedlings was optimized from two aspects:First,high concentration and low concentration hormone medium were used to optimize the quality of tissue culture seed⁃lings at the stage of proliferation;Second,the rooting quality of Chinese fir tissue culture seedlings was optimized by changing the light source during rooting stage.The results show that the proliferative buds treated with high concentration hormone and low concen⁃tration hormone has high proliferation rate,fast growth,moderate knot spacing and high lignification degree et al.With the increase of subculturing times,these characters are relatively stable.The rooting rate reached 86.5%and the rooting number reached 2.7 after the proliferative buds were planted to the rooting medium.The rooting rate reached 86.7%when the red-blue light ratio was 2∶1;The number of rooting reached 2.8 when the red-blue light ratio was 4∶1.

关 键 词：杉木[Cunninghamia lanceolata(Lamb.)Hook.] 组培苗 激素 LED复合光源 

分 类 号：S791.27[农业科学—林木遗传育种] Q813.12[农业科学—林学]