狗枣猕猴桃AkMYB5基因的生物信息学分析  

Bioinformatics analysis of AkMYB5 gene from Actinidia kolomikta(Maxim.et Rupr.)Maxim

作　　者：刘丹[1] 李然红[1] 陈鑫[1] 王立凤[1] LIU Dan;LI Ran-hong;CHEN Xin;WANG Li-feng(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157012,Heilongjiang,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157012

出　　处：《湖北农业科学》2020年第11期134-137,共4页Hubei Agricultural Sciences

基　　金：黑龙江省教育厅项目(1354MSYYB006;1354MSYYB007);黑龙江省教育厅备案项目(1351MSYYB007);北方药食技术工程中心专项(PT2018011)。

摘　　要：为研究狗枣猕猴桃[Actinidia kolomikta(Maxim. et Rupr.)Maxim.]AkMYB5的功能,利用生物信息学工具和方法,对其编码蛋白质的理化性质、亲水/疏水性、跨膜结构、结构功能域、二级结构、三级结构、蛋白质修饰位点及同源性等进行分析和预测。结果表明,AkMYB5的cDNA全长为1 339 bp,CDS为1 053 bp,编码蛋白由351个氨基酸构成的亲水性非稳定蛋白,属于MYB5蛋白家族(PLN03212),主要构件为α-螺旋和不规则卷曲,与其他18种植物的MYB氨基酸序列进行比对发现,其与中华猕猴桃变种(Actinidia chinensis var. chinensis)AkMYB5的亲缘关系较近。To obtain information about AkMYB5 gene from Actinidia kolomikta(Maxim.et Rupr.)Maxim.,the bioinformatics methods and tools were used to analysis the physical and chemical properties,hydrophilicity/hydrophobicity,transmenbranne domain,structure and function domain,secondary structure,tertiary structure,protein modification sites and homology of MYB5 from Actinidia kolomikta.The results showed that the length of AkMYB5 cDNA was 1339 bp and the coding area was 1053 bp coding 351 amino acid.AkMYB5 was a unstable and hydrophilic protein belonging to the MYB5 superfamily(PLN03212).The analysis also demonstrated that AkMYB5 main component was Alpha-helix and random coil.Compaired to MYBs of the other 18 plants,AkMYB5 had near relationships with Ac⁃tinidia chinensis var.chinensis.

关 键 词：狗枣猕猴桃[Actinidia kolomikta(Maxim.et Rupr.)Maxim.] AkMYB5 生物信息学 

分 类 号：S663.4[农业科学—果树学] Q75[农业科学—园艺学]