电针神庭、印堂穴对谵妄状态大鼠下丘脑5-羟色胺及白细胞介素-1β的影响  

Effect of Electroacupuncture at Shenting and Yintang Points on 5-HT and IL-1βin Hypothalamus of Rats in Delirium State

作　　者：吴擎 徐英 刘美真 吴燕 WU Qing;XU Ying;LIU Meizhen;WU Yan

机构地区：[1]宁波市第一医院,浙江宁波315010 [2]宁波市中医院,浙江宁波315010

出　　处：《新中医》2020年第12期126-129,共4页New Chinese Medicine

基　　金：宁波市科技计划项目(2018A610414)。

摘　　要：目的:观察电针对谵妄状态大鼠下丘脑5-羟色胺(5-HT)及白细胞介素-1β(IL-1β)表达的影响,从神经递质的角度探讨电针对东莨菪碱诱导的谵妄模型大鼠的镇静作用机制。方法:将60只2月龄SD大鼠,随机分为空白组、模型组、右美托咪定(DEX)组、电针组4组,每组15只。除空白组外,其余3组大鼠采用腹腔注射东莨菪碱(1.8 mg/kg)建立谵妄模型,DEX组及电针组在建立模型后立即腹腔注射DEX(30μg/kg),电针组同时针刺神庭、印堂穴,每次30 min,连续治疗7 d。对4组大鼠进行旷场实验;采用ELISA法检测下丘脑组织中5-HT、IL-1β水平。结果:与空白组比较,模型组穿格次数、垂直运动数、修饰行为次数及时间均减少(P<0.05),下丘脑5-HT、IL-1β含量均增加(P<0.05)。与模型组比较,DEX组、电针组穿格次数、垂直运动数、修饰行为次数及时间均增加(P<0.05,P<0.01),下丘脑5-HT、IL-1β含量均显著降低(P<0.05)。与DEX组比较,电针组穿格次数、垂直运动数、修饰行为次数及时间均增加(P<0.05),下丘脑5-HT、IL-1β含量均降低(P<0.05)。结论:电针针刺神庭、印堂穴可达到镇静的作用,且与DEX有协同作用,其作用机制可能是降低大鼠下丘脑内神经递质5-HT及IL-1β的含量。Objective:To observe the effect of electroacupuncture on the expression of 5-hydroxytryptamine(5-HT)and interleukin-1β(IL-1β)in hypothalamus of rats in delirium state,and discuss the mechanism of electroacupuncture on the rat models with delirium induced by scopolamine in terms of neurotransmitter.Methods:A total of 60 SD rats aged 2 months were randomly divided into the blank group,the model group,the dexmedetomidine(DEX)group and the electroacupuncture group,15 rats in each group.Except in the blank group,the rat models with delirium were established by intraperitoneal injection of scopolamine(1.8 mg/kg)in the other three groups;after model establishment,the DEX group and the electroacupuncture group were immediately given intraperitoneal injection of DEX(30μg/kg);meanwhile,the electroacupuncture group was given acupuncture at Shenting(GV 24)and Yintang(GV 29)points,30 minutes each time.All the groups except for the blank group were treated for 7 consecutive days.The four groups were given open field test;the levels of 5-HT and IL-1βin hypothalamic tissues were evaluated via ELISA method.Results:Compared with those in the blank group,the frequency and time of passing through the grid,vertical motion,and grooming behavior in the model group were decreased(P<0.05),and the contents of 5-HT and IL-1βin hypothalamus were increased(P<0.05).Compared with those in the model group,the frequency and time of passing through the grille,vertical motion and grooming behavior in the DEX group and the electroacupuncture group were increased(P<0.05,P<0.01),and the contents of 5-HT and IL-1βin hypothalamus were significantly decreased(P<0.05).Compared with those in the DEX group,the frequency and time of passing through the grille,vertical motion and grooming behavior in the electroacupuncture group were increased(P<0.05),and the contents of 5-HT and IL-1βin hypothalamus were decreased(P<0.05).Conclusion:Electroacupuncture at Shenting(GV 24)and Yintang(GV 29)points has sedative effect and synergistic effect with DEX.Its m

关 键 词：谵妄 电针 5-羟色胺 白细胞介素-1Β 动物实验 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]