补肾壮筋汤含药血清介导的Sox9基因高表达对IL-1β诱导的大鼠软骨细胞损伤的保护机制研究  

Protective Mechanism of Bushen Zhuangjin Decoction Containing Serum on IL-1β-Induced Chondrocyte Injury in Rats by Overexpression of Sox9

作　　者：吴锋锋[1] 高宏梁[1] 王国荣[1] 李建有[1] 黄胜[1] 蒋雪生[1] 李雄峰[1] Wu Fengfeng;Gao Hongliang;Wang Guorong(Department of Orthopedics and Rehabilitation,Huzhou Central Hospital,Zhejiang 313000,China)

机构地区：[1]湖州市中心医院(浙江大学湖州医院)骨科与康复科,313000

出　　处：《医学研究杂志》2020年第7期96-99,共4页Journal of Medical Research

基　　金：浙江省中医药管理局科技项目(2016ZA185)。

摘　　要：目的评估补肾壮筋汤(BZD)含药血清介导的Sox9基因高表达对IL-1β诱导的大鼠软骨细胞损伤的保护作用。方法传代培养的第2代大鼠软骨细胞随机分为3组,即对照组不予任何干预,模型组给予IL-1β(10μg/ml)刺激软骨细胞24h,BZD组予IL-1β(10μg/ml)刺激软骨细胞24h后,加入BZD含药血清(400μg/ml)。BZD干预72h后收集所有软骨细胞进行分析。结果与对照组比较,模型组软骨细胞活性明显下降(P=0.000),软骨细胞Sox9、蛋白聚糖aggrecan及胶原蛋白Ⅱ的基因及蛋白水平均明显下降(P=0.000);与模型组比较,BZD组增强了软骨细胞活性(P<0.05),软骨细胞Sox9、蛋白聚糖aggrecan及胶原蛋白Ⅱ的基因及蛋白水平均明显上升(P<0.05)。结论BZD在IL-1β诱导的大鼠软骨细胞损伤中可能通过过表达Sox9起保护作用。Objective Over expression of Sox9 has been shown to promote cartilage repair in early stages of human osteoarthritis.This study aimed to determine whether Bushen Zhuangjin decoction(BZD)protected chondrocytes against IL-1β-induced injury by elevating Sox9 expression.Methods P2 chondrocytes were randomly divided into three groups:a control group,a model group stimulated with IL-1β(10μg/ml)for 24h,and a BZD group stimulated with IL-1βfor 24h and then treated with serum containing BZD(400μg/ml)for 72h.Chondrocytes in all groups were collected at the same time(72h after BZD administration in the BZD group)for analysis.Results Compared with the control group,IL-1βtreatment significantly reduced cell viability(P=0.000).Both mRNA and protein expression levels of SOX9,aggrecan,and collagenⅡwere significantly down regulated in the model group compared to those of the control group(P=0.000).However,BZD significantly alleviated the inhibitory effect of IL-1βon chondrocyte viability(P<0.05).Moreover,in the BZD group,both the mRNA and protein expression levels of SOX9,aggrecan,and collagenⅡwere significantly up regulated compared with those in the model group(P<0.05).Conclusion This study demonstrated that BZD could protect chondrocytes against IL-1β-induced injury by elevating Sox9 expression.

关 键 词：补肾壮筋汤 IL-1Β 骨关节炎 SOX9 胶原蛋白Ⅱ 

分 类 号：R681[医药卫生—骨科学]