云南省蓝舌病病毒“历史毒株”的遗传特征  

Genetic Characteristics of“Historical Strains”of Bluetongue Virus Isolated from Yunnan Province

作　　者：李卓然 朱建波[1] 廖德芳[1] 肖雷[1] 王金萍[1] 高林[1] 杨振兴[1] 李占鸿 杨恒[1] 李华春[1] Li Zhuoran;Zhu Jianbo;Liao Defang;Xiao Lei;Wang Jinping;Gao Lin;Yang Zhenxing;Li Zhanhong;Yang Heng;Li Huachun(Key Laboratory of Tropical and Subtropical Animal Virus,Yunnan Academy of Animal and Veterinary Science,Kunming,Yunnan 650224,China)

机构地区：[1]云南省畜牧兽医科学院,云南省热带亚热带动物病毒病重点实验室,云南昆明650224

出　　处：《中国动物检疫》2020年第8期26-35,42,共11页China Animal Health Inspection

基　　金：国家重点研发计划项目(2017YFC1200505);国家自然科学基金项目(31760744);公益性行业(农业)科研专项(201303035);云南省中青年学术和技术带头人后备人才培养项目(2017HB055);云南省基础研究计划项目(2019FB041)。

摘　　要：20世纪90年代,本实验室从云南省哨兵动物采集的血液中分离到7种血清型蓝舌病病毒(bluetongue virus,BTV)。但这些毒株的遗传特征至今仍不清楚,因而阻碍了我国的BTV演化历史分析。为掌握云南省早期流行BTV毒株的遗传特征,对1995—1997年云南省分离的25株BTV毒株的基因组节段2、3、7和10(Seg-2、-3、-7、-10)进行一步法RT-PCR扩增、测序以及序列分析。Seg-2序列分析显示,1995—1997年云南省分离到的7种不同血清型(BTV-1、-2、-3、-4、-12、-15、-16)BTV毒株,分属A、B、G、H、I和J等6种基因型;BTV-12型毒株的Seg-2为西方地域型,其余毒株的Seg-2则属于东方地域型;Seg-3和Seg-10序列分析显示,25株BTV毒株均为东方地域型;Seg-7序列分析显示,1株BTV-2型、2株BTV-12型和1株BTV-16型毒株属于西方地域亚型,并与南非和荷兰BTV毒株的Seg-7具有最近的亲缘关系,其余毒株则均为东方地域型。上述结果表明,云南省存在多种血清型BTV的流行,而Seg-2和Seg-7基因重配毒株的发现,提示国外的西方地域型毒株已侵入云南省,并在传播过程中与我国毒株发生了基因重配。本研究为我国BTV的演化分析与溯源研究提供了数据参考。In the 1990 s,7 serotypes of bluetongue virus(BTV)were isolated from blood samples collected from sentinel animals in Yunnan Province by our laboratory.However,the genetic characteristics of these strains were still not clear,which hindered the study on the evolutionary history of BTV in China.In order to make clear the genetic characteristics of BTV strains spread early in Yunnan,one-step RT-PCR amplification,sequencing and sequence analysis were carried out for gene segments 2,3,7 and 10(Seg-2,-3,-7 and-10)of 25 BTV strains isolated in the province from 1995 to 1997.It was found that,by Seg-2 sequence analysis,Seven different BTV serotype strains(BTV-1,-2,-3,-4,-12,-15 and-16)were isolated,which fell into 6 nucleotypes of A,B,G,H,I and J,respectively;the Seg-2 of BTV-12 fell into Western topotype,but other strains belonged to Eastern topotype;it was showed that,by Seg-3 and Seg-10 sequence analysis,25 strains of BTV belonged to Eastern topotype;and by Seg-7 sequence analysis,one strain of BTV-2,two strains of BTV-12 and one strain of BTV-16 were clustered into Western topotype,and shared the closest genetic relationship with Seg-7 isolated from South Africa and Netherlands,and the other strains belonged to Eastern topotype.It was concluded that multiple serotypes of BTV were prevalent in Yunnan Province,and foreign Western topotype strains had been introduced into the province and reassorted with Chinese strains in the process of transmission as Seg-2 and Seg-7 gene reassortative isolates were detected.Some data was provided for evolution analysis and traceability study on BTV in China.

关 键 词：蓝舌病病毒 序列分析 遗传特征 基因重配 地域型 血清型 

分 类 号：S852.65[农业科学—基础兽医学]