布鲁氏菌S2疫苗阴道途径免疫羊的安全性初步研究  

Preliminary Study on the Safety of Brucella Vaccine S2 Strain on Goats Immunized via Vagina

作　　者：韩焘[1] 毕一鸣[1] 穆佳毅 彭小薇[2] 蒋卉[2] 冯宇[2] 于广秋 郭富存 左海莉 王传彬[1] 董浩[1] Han Tao;Bi Yiming;Mu Jiayi;Peng Xiaowei;Jiang Hui;Feng Yu;Yu Guangqiu;Guo Fucun;Zuo Haili;Wang Chuanbin;Dong Hao(China Animal Disease Control Center,Beijing 100125,China;China Institute of Veterinary Drug Control,Beijing 102618,China;Jianchang Animal Husbandry and Veterinary Bureau,Jianchang,Liaoning 125300,China;Jiuyuan District Animal Disease Control Center,Baotou,Inner Mongolia 014060,China)

机构地区：[1]中国动物疫病预防控制中心,北京100125 [2]中国兽医药品监察所,北京102618 [3]建昌县畜牧兽医局,辽宁建昌125300 [4]包头市九原区动物疫病预防控制中心,内蒙古包头014060

出　　处：《中国动物检疫》2020年第8期88-92,共5页China Animal Health Inspection

基　　金：国家自然科学基金项目(31602055)。

摘　　要：布鲁氏菌病(简称布病)是一种由布鲁氏菌引起的严重危害人和动物健康的人兽共患病。疫苗免疫是畜间布病流行率较高地区控制该病的重要手段。辽宁省建昌县经过多年实践,创新出一套免疫保护效果较好的阴道途径免疫S2疫苗的新型家畜免疫技术。为了解该技术对家畜的安全性,本研究采用该技术免疫了3只山羊,在免疫1个月后检测其抗体滴度、体内含菌量,观察脾脏和肝脏的病理变化。结果显示,3只免疫山羊均抗体阳转,所有采集的脏器均未分离到布鲁氏菌,且脾脏和肝脏未出现布病相关病理变化。初步研究表明,通过阴道途径对羊进行S2疫苗免疫是安全的。Brucellosis is a kind of zoonosis caused by Brucella,which seriously endangers human and animal health,and is mainly controlled through vaccination in areas where animal brucellosis is prevalent.After practices for several years,a new immune technology(immunization via vagina)was developed in Jianchang County of Liaoning Province,and good immune effects have been achieved.In order to make clear the safety of the technology on animals,3 goats were vaccinated,one month later,their antibody titer and bacterial content were detected and the pathological changes of their spleens and livers were observed.The results showed that the antibodies in the three goats turned to be positive,no Brucella was isolated from all the organs collected,and no pathological changes related to brucellosis were found in spleens and livers.These results preliminarily indicated that it was safe to immunize goat with Brucellavaccine S2 strain via the vagina.

关 键 词：布鲁氏菌病 疫苗 阴道免疫 安全性 

分 类 号：S851.3[农业科学—预防兽医学]