限根栽培对万寿菊根际微生物区系的影响  

Effects of Root-limited Cultivation on Microbial Flora of Marigold Rhizosphere Soil

作　　者：陈泽斌 李希杨 阮亚男 徐胜光 CHEN Zebin;LI Xiyang;RUAN Yanan;XU Shengguang(College of Agriculture and Life Sciences,Kunming University,Kunming,Yunnan,China 650214)

机构地区：[1]昆明学院农学与生命科学学院,云南昆明650214

出　　处：《昆明学院学报》2020年第3期60-63,共4页Journal of Kunming University

基　　金：国家自然科学基金资助项目(41361056);云南省地方本科高校基础研究联合专项资助项目(2017FH001-041).

摘　　要：为探讨限根栽培对万寿菊根际土壤微生物种类和丰度的影响.采用二代测序技术测定万寿菊根际土壤DNA中16S rDNA的V4-5和ITS1区.结果表明,限根栽培条件下根际土壤细菌类群的辛普森指数、香农指数、OTU丰度略低于非限根栽培,表明限根栽培略降低了细菌OTU的丰富度.而真菌类群的辛普森指数、香农指数和OTU丰度均高于非限根栽培,说明限根栽培提高了真菌类群OTU的丰富度.In order to study the effects of root-limited cultivation on microbial species and abundance in marigold rhizosphere soil,the V4-5 and ITS1 regions of 16S rDNA in marigold rhizosphere soil were determined by second-generation sequencing.The results showed that the Simpson index,Shannon index and OTU abundance of bacteria in rhizosphere soil were slightly lower than those in nonrhizosphere cultivation,indicating that root-limited cultivation slightly reduced the bacterial OTU richness,but Simpson index,Shannon index and OTU abundance of fungi were higher than those of non-root-limited cultivation,indicating that root-limited cultivation improved the OTU abundance of fungi.

关 键 词：限根栽培 土壤 细菌 真菌 多样性 

分 类 号：S682.1[农业科学—观赏园艺]