LncRNA KCNQ1OT1在人脂肪细胞分化过程及肥胖人群脂肪组织中的表达  

Expression of lncRNA KCNQ1OT1 in human adipocyte differentiation and adipose tissue of obese people

作　　者：陈辰[1] 王琰 张卓[2] 张晓笑 季晨博[2] 池霞[3] 尤梁惠 Chen Chen;Wang Yan;Zhang Zhuo;Zhang Xiaoxiao;Ji Chenbo;Chi Xia;You Lianghui(Department of Clinical Laboratory,Women′s Hospital of Nanjing Medical University(Nanjing Maternity and Child Health Care Hospital),Nanjing 210004,China;Nanjing Maternity and Child Health Care Institute,Women′s Hospital of Nanjing Medical University(Nanjing Maternity and Child Health Care Hospital),Nanjin 210004,China;Department of Child Health Care,Women′s Hospital of Nanjing Medical University(Nanjing Maternity and Child Health Care Hospital),Nanjing 210004,China)

机构地区：[1]南京医科大学附属妇产医院(南京市妇幼保健院)检验科,210004 [2]南京医科大学附属妇产医院(南京市妇幼保健院)医学研究中心,210004 [3]南京医科大学附属妇产医院(南京市妇幼保健院)儿保科,210004

出　　处：《中华内分泌代谢杂志》2020年第6期469-473,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金(81600687)。

摘　　要：目的探讨长链非编码RNA(long noncoding RNA,lncRNA)KCNQ1OT1在脂肪前体细胞分化过程及肥胖者、脂肪组织中的表达变化,阐明KCNQ1OT1与肥胖的相关性,为进一步解读lncRNA参与肥胖发生发展的作用提供线索。方法用PPIA为内参基因,通过定量PCR,检测KCNQ1OT1于人脂肪前体细胞分化第0、1、3、5、9、12天的表达水平;应用定量PCR检测KCNQ1OT1在肥胖和正常人群白色脂肪组织中的表达变化;采用Pearson相关分析探讨KCNQ1OT1与人群体重指数、血清三酰甘油及总胆固醇的相关性。结果KCNQ1OT1在脂肪前体细胞分化第1、3、5、9和12天的相对表达量分别为(25.89±3.10)、(24.78±5.58)、(15.53±2.11)、(6.75±0.71)、(4.81±0.84),与第0天相比,均呈上升趋势,差异有统计学意义(P<0.01),在分化初始增长尤为明显(第1、3天)。KCNQ1OT1在肥胖人群内脏脂肪组织中的相对表达量为0.79±0.05,较正常人显著增加(P<0.01)。KCNQ1OT1与体重指数呈正相关(r=0.569,P<0.01),与三酰甘油含量呈正相关(r=0.489,P<0.05),与总胆固醇含量呈正相关(r=0.591,P<0.01)。结论KCNQ1OT1在人脂肪前体细胞分化过程中呈增高趋势;在肥胖人群脂肪组织中的表达增高,其表达与体重指数、血清三酰甘油等肥胖相关指标呈正相关,提示KCNQ1OT1可能是影响人脂肪细胞分化的重要调控因子及肥胖防治的潜在靶标。Objective This study was to investigate the expression of long noncoding RNA(lncRNA)KCNQ1OT1 during the differentiation program of human preadipocyte and to look for the changes of its expression in adipose tissue in obese subject,as well as to clarify the correlation between KCNQ1OT1 and obesity,and to provide clues for further understanding the role of lncRNA in the development of obesity.Methods Quantitative PCR was used to detect the expression of KCNQ1OT1 in human preadipocyte at day 0,1,3,5,9,and 12 during differentiation program,quantitative PCR was also used to detect KCNQ1OT1 expression in white adipose tissue of obese and normal people,which related to PPIA internal reference gene.Pearson correlation analyses were used to explore the relationships of KCNQ1OT1 with body mass index,triglyceride,and total cholesterol.Results During differentiation program,the relative expression of KCNQ1OT1 levels at day of 1,3,5,9,and 12 were(25.89±3.10),(24.78±5.58),(15.53±2.11),(6.75±0.71),(4.81±0.84),which showed an upward trend compared with day 0.This difference was significant(P<0.01),especially in the early stage of differentiation(day 1 and day 3).The relative expression of KCNQ1OT1 in visceral adipose tissue of obese subjects was 0.79±0.05,which was significantly higher than that of normal people(P<0.01).KCNQ1OT1 was positively correlated with body mass index(r=0.569,P<0.01),triacylglycerol content(r=0.489,P<0.05),and total cholesterol content(r=0.591,P<0.01).Conclusion KCNQ1OT1 expression level,which was up-regulated in adipose tissue from obese subjects,increased during the differentiation program of preadipocytes,and also positively correlated with body mass index and serum triglyceride content.These results suggest that KCNQ1OT1 may be an important regulator of human preadipocyte differentiation and a potential target for prevention and treatment of obesity.

关 键 词：KCNQ1OT1 LncRNA 人脂肪前体细胞 诱导分化 肥胖 

分 类 号：R589.2[医药卫生—内分泌]