地塞米松对大鼠心肌梗死后巨噬细胞极化及心功能的影响  

Effects of dexamethasone on macrophage polarization and cardiac function after myocardial infarction in rats

作　　者：宋吉哲 刘成林 杜爱玲[2] SONG Jizhe;LIU Chenglin;DU Ailing(Department of Internal Medicine,Weifang Medical College,Weifang 261000,China)

机构地区：[1]潍坊医学院内科学教研室,山东潍坊261000 [2]潍坊医学院附属医院心内科,山东潍坊261031

出　　处：《实用医学杂志》2020年第14期1892-1896,共5页The Journal of Practical Medicine

基　　金：山东省医药卫生科技发展计划项目(编号:2017WS406);山东省中医药科技发展计划项目(编号:2017-224)。

摘　　要：目的观察地塞米松对心肌梗死大鼠巨噬细胞极化、炎症反应及心功能的影响。方法将44只雄性SD大鼠分为3组:假手术组、对照组、实验组,其中假手术组采用随机等间隔抽样法抽取8只,其余36只大鼠建立AMI模型,24 h后将建模成功存活的19只大鼠随机分为对照组10只和实验组9只,实验组大鼠在建模后24 h肌肉注射1次地塞米松磷酸钠注射液(1 mg/kg),对照组肌肉注射1次生理盐水(0.2 mL/kg)。术后4周,对所有存活的大鼠行超声心动图检测心功能;HE染色法检测梗死区心肌组织形态学改变;RT-PCR检测心肌梗死区周围组织M1型巨噬细胞标志物IL-6、TNF-α和M2型巨噬细胞标志物IL-10、TGF-β1的mRNA表达水平。结果 (1)AMI模型大鼠24 h存活率低于假手术组(P <0.05);实验组与对照组大鼠4周生存率比较差异无统计学意义(P> 0.05)。(2)对照组和实验组大鼠LVESD、LVEDD大于假手术组(P <0.05),LVEF及FS低于假手术组(P <0.05);实验组LVESD及LVEDD小于对照组(P <0.05),LVEF及FS高于对照组(P <0.05)。(3)HE染色示假手术组心肌组织细胞排列均匀,无明显病理改变,实验组的心肌细胞结构优于对照组。(4)RT-PCR示:对照组及实验组大鼠梗死区周围组织TNF-α、IL-6、IL-10和TGF-β1的mRNA相对表达量显著高于假手术组(均P <0.05);与对照组比较,实验组大鼠M1型巨噬细胞标志物TNF-α和IL-6的mRNA相对表达量显著降低(均P <0.05),M2型巨噬细胞标志物IL-10和TGF-β1的mRNA相对表达量显著升高(均P <0.05)。结论地塞米松可减轻大鼠急性心肌梗死区的心肌组织损伤及改善心功能;其机制可能与地塞米松通过调节M1型巨噬细胞向M2型极化,抑制心肌组织炎症反应及梗死后心室重构有关。Objective To investigate the effects of dexamethasone on macrophage polarization,inflamma-tory response and cardiac function in rats with myocardial infarction.MethodsA total of 44 male SD rats weredivided into 3 groups:sham operation group,control group and experimental group. Among the sham operationgroup,8 rats were sampled by random equal interval sampling. The remaining 36 rats were established with AMImodel. After 24 hours,19 surviving rats were randomly divided into control group(n = 10)and experimental group(n = 9). Rats in the experimental group were injected with dexamethasone(1 mg/kg)after the successful model-ing. Rats in the control group were injected with normal saline(0.2 m L/kg)once. Four weeks after the operation,echocardiography was performed to detect the cardiac function of all the surviving rats. HE staining was used todetect the morphological changes of myocardial tissue in the infarcted area. RT-PCR was used to detect the m RNAexpression levels of M1 macrophage markers IL-6 and TNF-α,M2 macrophage markers IL-10 and TGF-β1 in thetissues around the infarcted area.Results(1)The 24-hour survival rate of AMI model rats was lower than that ofthe sham operation group(P < 0.05). The 4-week survival rate of the experimental group and the control group wasnot statistically different(P > 0.05).(2)LVESD and LVEDD in the control group and the experimental group werehigher than those in the sham operation group(P < 0.05),while LVEF and FS were lower than those in the shamoperation group(P < 0.05). LVESD and LVEDD in experimental group were lower than that in control group(P <0.05),while LVEF and FS were higher than that in control group(P < 0.05).(3)HE staining showed that themyocardial tissue cells in the sham operation group were evenly arranged without obvious pathological changes,and the myocardial cell structure in the experimental group was better than that in the control group.(4)RT-PCRshowed that the relative m RNA expression levels of TNF-α,IL-6,IL-10 and TGF-β1 in the control group and the

关 键 词：急性心肌梗死 糖皮质激素 炎症 巨噬细胞极化 大鼠 

分 类 号：R541.4[医药卫生—心血管疾病]