利用液质联用技术同时检测血清19种维生素的方法  

Simultaneous detection of serum levels of 19 vitamins based on LC-MS/MS

作　　者：林东子 刁嘉茵 雷春燕 王威 肖妮 李玉美 林炳耀 杨月明 孙志豪[4] 张召 LIN Dong-zi;DIAO Jia-yin;LEI Chun-yan;WANG Wei;SHAO Ni;LI Yu-mei;LIN Bing-yao;YANG Yue-ming;SUN Zhi-hao;ZHANG Zhao(Foshan Fourth People's Hospital,Foshan 528000,China;Guangzhou Nanxin Medical Laboratory Co.Ltd.,Guangzhou 524000,China;Dongguan Sixth People's Hospital,Dongguan 523008,China;Dongguan Maternal and Child Health Hospital,Dongguan 523170,China;Biomedicine Institute of South China,Guangzhou 524000,China)

机构地区：[1]佛山市第四人民医院,广东佛山528000 [2]广州南芯医学检验实验室有限公司,广东广州524000 [3]东莞市第六人民医院,广东东莞523008 [4]广东省东莞市妇幼保健院,广东东莞523170 [5]华南生物医药研究院,广东广州524000

出　　处：《广东医科大学学报》2020年第4期400-406,共7页Journal of Guangdong Medical University

基　　金：广州市科技计划项目(No.202002030200);广东省医学科研基金(No.B2018014);东莞市社会科技发展项目(No.2018507150051657);佛山市自筹经费类科技计划项目(医学类科技攻关),(No.1920001000648);佛山市卫生健康局医学科研课题(No.20200224,No.20180138)。

摘　　要：目的建立测定人血清19种(13种水溶性和6种脂溶性)维生素质量浓度的高效液相串联质谱(LC-MS/MS)法。方法人血清样本中分别用亲水柱、T3反相柱提取水溶性、脂溶性维生素,电喷雾正负离子模式下以多反应检测方式检测,内标法定量。结果19种维生素标准曲线的线性相关系数为0.994~0.999,精密度RSD为0%~15%,回收率RSD为85%~115%,最低检出限为0.0005~21.42μg/L。结论该方法同时测定血清19种维生素,具有重现性好、精确度高、通量高等优点。Objective To establish a high performance LC-MS/MS method for detecting serum contents of 19(13 waterand 6 lipid-soluble)vitamins in humans.Methods Water-and lipid-soluble vitamins were extracted from serum samples using hydrophilic column and T3 reversed-phase column,respectively.Positive and negative electrospray ionization was performed by multiple reaction monitoring mode.The internal standard was used for quantification.Results The linear correlation coefficients of 19 vitamins were 0.994-0.999.The relative standard deviation(RSD)values of precision and recovery were 0-15%and 85%-115%,respectively.The minimum detection limits were 0.0005-21.42μg/L.Conclusion The method is reproducible,accurate,and high-throughput?for simultaneous detection of serum levels of 19 vitamins.

关 键 词：超高相液相串联质谱 维生素 定量检测 

分 类 号：Q56[生物学—生物化学]