重组酶介导的等温扩增技术及其在植物病原检测中的应用  

Recombinase polymerase amplification and its applications in plant pathogens detection

作　　者：周莹[1] 岳瑾 李云龙 刘梅[1] 乔广行[1] Zhou Ying;Yue Jin;Li Yunlong;Liu Mei;Qiao Guanghang(Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Beijing Plant Protection Station,Beijing 100029,China)

机构地区：[1]北京市农林科学院植物保护环境保护研究所,北京100097 [2]北京市植物保护站,北京100029

出　　处：《中国植保导刊》2020年第6期27-31,共5页China Plant Protection

基　　金：北京市农林科学院青年科研基金项目(QNJJ201705);北京市农林科学院创新能力建设项目(KJCX20180705)。

摘　　要：重组酶介导的等温扩增技术(Recombinase polymerase amplification, RPA)是一种新型的恒温核酸体外扩增技术,与传统PCR和其他等温扩增技术相比较,具有对仪器依赖性低、核酸扩增效率高的优势,适合于现场快速检测,已在微生物检测领域有了较广泛的应用。本文介绍了RPA的技术原理、产物检测方法以及技术条件的摸索优化,并概述了近年来此技术在植物病原检测领域的应用情况,展望了今后RPA技术的发展方向,以期为新兴技术在植物病原检测中的进一步应用与改进研究提供参考。Recombinase polymerase amplification(RPA)is a recently developed isothermal nucleic acid amplification technology.Compared with PCR and other isothermal DNA amplification technology,the RPA reaction does not require specialized equipment but with high efficiency of nucleic acid amplification.It is suitable for quick detection and has been widely used in the field of microbial detection.This article reviewed the technical principle of RPA,as well as the product detection method,the reaction condition optimization,and the research achievements of detection in plant pathogens,in expectation of providing a reference for the development of such emerging technologies in the future.

关 键 词：等温扩增技术 植物病原 检测 

分 类 号：S432.4[农业科学—植物病理学]