玛瑙膏质量标准研究  

Quality standard for Ma′nao Gao

作　　者：李筱婧 韦英杰[1,2] 汪晶[1,2] 许欢 黄一平[1,2] 江阿古丽·艾山 陈斌[1,2] LI Xiaojing;WEI Yingjie;WANG Jing;XU Huan;HUANG Yiping;Janagul·As;CHEN Bin(The Third Clinical Medical College,Nanjing University of Chinese Medicine,Jiangsu Province,Nanjing210028,China;Key Laboratory for Traditional Chinese Medicine Drug Release System Under State Administration of Traditional Chinese Medicine,Jiangsu Province Academy of Traditional Chinese Medicine,Jiangsu Province,Nanjing210028,China;Xinjiang Yili Kazak Autonomous Prefecture Hospital of Traditional Chinese Medicine,Xinjiang Uygur Autonomous Region,Yining835000,China)

机构地区：[1]南京中医药大学第三临床医学院,江苏南京210028 [2]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,江苏南京210028 [3]新疆伊犁哈萨克自治州中医医院,新疆伊宁835000

出　　处：《中国医药导报》2020年第21期133-136,F0004,共5页China Medical Herald

基　　金：江苏省医学创新团队项目(CXTDB2017003);江苏省研究生培养创新工程项目(SJCX19_0375)。

摘　　要：目的建立玛瑙膏的质量标准。方法采用薄层色谱法对制剂中五灵脂、延胡索、大黄、补骨脂、三七进行定性鉴别;采用高效液相色谱法对制剂中延胡索乙素进行含量测定,以乙腈-0.1%磷酸三乙胺体系为流动相,梯度洗脱,柱温30℃,检测波长280 nm。结果薄层色谱法斑点清晰,分离度好,阴性无干扰;延胡索乙素在2.022~20.22μg/mL(r=0.9999)范围内呈良好的线性关系,平均加样回收率为100.27%(RSD=2.39%)。结论本研究建立的方法稳定性、重复性好,可用于玛瑙膏的质量控制。Objective To establish a quality control method for Ma′nao Gao.Methods Thin layer chromatography was applied to the qualitative identification of Faeces Togopteri,Corydalis Rhizoma,Rhei Radix et Rhizoma,Psoraleae Fructus,Notoginseng Radix et Rhizoma.High performance liquid chromatography was used for the content determination of tetrahydropalmatine,acetonitrile-0.1%triethylamine phosphate system was used as the mobile phase of gradient elute.The column temperature was 30°C and the detection wavelength was set at 280 nm.Results Thin layer chromatography spots was clear,which had good resolution without interference.Tetrahydropalmatine had good linear relationship with the range of 2.022-20.22μg/mL(r=0.9999)respectively,the average recovery was 100.27%(RSD=2.39%).Conclusion The method is reliable with good stability and good repeatability,and can be used for quality control of Ma′nao Gao.

关 键 词：玛瑙膏 质量标准 薄层色谱法 高效液相色谱法 延胡索乙素 

分 类 号：R927.1[医药卫生—药学]