稳定同位素指纹分析在车厘子产地溯源中的应用  

Application of Stable Isotope Fingerprint Analysis in the Origin Tracing of Cherries

作　　者：金俊 郑方媛 周秀雯 潘家荣 JIN Jun;ZHENG Fangyuan;ZHOU Xiuwen;PAN Jiarong(College of Life Sciences,China Jiliang University/Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province/National&Local United Engineering Lab of Quality Controlling Technology and Instrumentation for Marine Food,Hangzhou,Zhejiang 310018)

机构地区：[1]中国计量大学生命科学学院/浙江省海洋食品品质及危害物控制技术重点实验室/海洋食品加工质量控制技术与仪器国家地方联合工程实验室,浙江杭州310018

出　　处：《核农学报》2020年第8期1722-1728,共7页Journal of Nuclear Agricultural Sciences

基　　金：“十三五”国家重点研发计划项目(2017YFF0211300)。

摘　　要：为满足进出口食品产地溯源的贸易需求,本研究以不同产地车厘子为研究对象,利用单体分子蛋白质和脂肪的13C、2H和18O同位素组成,通过单因素方差分析、多重比较分析、聚类分析、Fisher线性判别分析、逐步判别分析和留一法交叉验证,建立车厘子产地溯源模型。结果表明,不同产地间车厘子果肉蛋白质或脂肪中δ13C、δ2H和δ18O均存在差异,大部分达到显著性水平;多种同位素指标耦合的产地判别效率显著优于单一同位素指标,且逐步判别分析结果表明,随着耦合指标的增加,产地初始判别率和交叉验证判别率逐步提高,但3个指标耦合后,再增加耦合指标,产地初始判别率和交叉验证判别率不再提高。最后利用蛋白质δ2H、δ18O和脂肪δ2H 3个指标建立了溯源模型,模型的初始判别率达到100.0%,交叉验证判别率为93.8%。本研究结果为进出口食品产地鉴别提供了一定的依据。In order to meet the demand for geographical origin tracing of imported and exported food across the borders,13C,2H and 18O isotopic compositions of protein and fat in pulp of cherries from different regions were determined in this study.One-way analysis of variance,multiple comparative analysis,cluster analysis,Fisher linear discriminant analysis,stepwise discriminant analysis and leave-one-way cross-validation analysis were used to establish cherries origin assessment.The results showed that there were differences inδ13 C、δ2H andδ18 O of protein and fat in the pulp of cherries among Australia,America,Chile and Shandong(China),and significant differences were found in most cases.The origin discrimination efficiency of multiple isotopic indicators was significantly better than that of single isotope.The results of stepwise discriminant analysis showed that with the increase of parameters combined,both of the initial discriminant rate and cross-validation discriminant rate of origin were gradually increased,which kept stable after four parameters.Finally,a mathematical model was established by using the combination of proteinδ2H,δ18O and fatδ2H in fruit pulp,which provided 100.0%and 93.8%of initial discriminant rate and the cross-validation discrimination rate,respectively.The results of this study provided a basis for origin the discrimination of imported and exported food.

关 键 词：车厘子 产地溯源 稳定同位素 蛋白质 脂肪 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]